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Volume 66, Issue 11

gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste Free

Abstract

A strictly anaerobic bacterial strain (WN011) was isolated from a methanogenic reactor treating waste from cattle farms. Cells of the strain were Gram-stain-negative curved rods with a polar flagellum. Spores were not produced. The optimum temperature for growth was 35–37 °C and the optimum pH was 6.7. The strain did not utilize carbohydrates as growth substrates. The strain grew in PY medium and produced acetate, butyrate, isovalerate and H as well as propionate and isobutyrate as minor products. Amino acids (-isoleucine, -leucine, -lysine, -serine, -threonine and -valine) added to PY medium enhanced growth of the strain and increased the amounts of fermentation products. Oxidase, catalase and nitrate-reducing activities were negative. Hydrogen sulfide was produced. The genomic DNA G+C content was 38.8 mol%. Compounds related to iso-C (fatty acid, dimethylacetal and aldehyde) were detected as predominant components by the cellular fatty acids analysis. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. On the basis of 16S rRNA gene sequences, three clones from wastewater were very closely related to strain WN011 (up to 99.9 % sequence similarity). The most closely related described species were those in cluster XIVa of the class such as (93.8 % 16S rRNA gene sequence similarity), (93.5 %) and (93.5 %). Based on the distinct differences in phylogenetic and phenotypic characteristics of strain WN011 from those of related species, it is concluded that strain WN011 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is WN011 (=JCM 16476=DSM 22923).

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Keyword(s): Amino acid decomposition , anaerobes , clostridial cluster XIVa , Falcatimonas natans and methanogenic reactor.
© 2016 IUMS
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2016-11-01
2024-04-18
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Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste
Int J Syst Evol Microbiol 66, 4639 (2016); https://doi.org/10.1099/ijsem.0.001403
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