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Volume 66, Issue 11

gen. nov., sp. nov., an anaerobic bacterium, isolated from a thermal spring and description of fam. nov. Free

Abstract

A mesophilic anaerobic bacterium, designated KHALHBd91 was isolated from the moderately hot spring of Hammam Biadha, Tunisia. The strain was Gram-staining-negative, non-sporulating, non-motile and rod-shaped, appearing singly (0.5–2.0×0.5–1 µm). It grew anaerobically at temperatures between 20 and 50 °C (optimum 37 °C) and at pH values between 5.5 and 7.8 (optimum 7.0). It required NaCl for growth, with growth observed at up 8.5 % and an optimum at 2.5 %. KHALHBd91 used glucose, galactose, maltose, pyruvate, lactate, fumarate and yeast extract as electron donors. The end-products from glucose fermentation were acetate, propionate, succinate and CO. Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate and sulfite were not used as terminal electron acceptors. The predominant cellular fatty acids were anteiso-C and iso-C. The respiratory quinone was MK-6. The main polar lipids consisted of lipids, phospholipids, glycolipids, aminolipids, phosphoaminoglycolipids and phosphatidylethanolamine. The DNA G+C content was 35.0 mol%. Phylogenetic analysis of the small-subunit ribosomal 16S rRNA gene sequence indicated that KHALHBd91 had and (phylum , class , order ) as its closest relatives (similarity of 86.7 and 87.8 % respectively). The phylogenetic and physiological data fro the present study strongly indicate that the isolate represents a novel genus and species of a novel family, gen. nov., sp. nov., in the family fam. nov. The type strain is KHALHBd91 (=DSM28579=JCM19909).

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Keyword(s): anaerobic , Balneicella halophila and hydrothermal spring
© 2016 IUMS
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2016-11-01
2024-04-19
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Balneicella halophila gen. nov., sp. nov., an anaerobic bacterium, isolated from a thermal spring and description of Balneicellaceae fam. nov.
Int J Syst Evol Microbiol 66, 4692 (2016); https://doi.org/10.1099/ijsem.0.001412
/content/journal/ijsem/10.1099/ijsem.0.001412
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