%0 Journal Article %A Li, Xiao-Jun %A Liu, Shao-Wei %A Jiang, Zhong-Ke %A Wu, Gang %A Sun, Cheng-Hang %T Hoyosella rhizosphaerae sp. nov., a halotolerant actinobacterium isolated from rhizosphere soil of Suaeda salsa, and emended description of the genus Hoyosella %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 11 %P 4716-4722 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001416 %K saline lake %K Hoyosella rhizosphaerae %K Suaeda salsa %K halotolerant actinobacterium %K Hoyosella %I Microbiology Society, %X A halotolerant actinobacterium, designated J12GA03T, was isolated from a rhizosphere soil sample of Suaeda salsa collected from a dried saline lake in Hebei Province, China. Cells were Gram-staining-positive, non-motile and non-spore-forming cocci. Strain J12GA03T grew optimally at 28‒37 °C, 0‒3 % NaCl (w/v) and pH 6.5‒7.5. It contained meso-diaminopimelic acid as the diagnostic diamino acid and arabinose, galactose and ribose as the diagnostic whole-cell sugars. MK-8 and MK-7 were detected as predominant menaquinones. Major fatty acids were C17 : 1 ω8c, C16 : 0 and C17 : 0. Polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphoglycolipids, glycolipids, unidentified phospholipids and additional lipids. The muramyl residue was acetyl. Mycolic acids (34–38 carbon atoms) were present. The G+C content of the genomic DNA was 55.8 mol%. It shared the highest 16S rRNA gene sequence similarities with Amycolicicoccus subflavus DQS3-9A1T (98.18 %) and Hoyosella altamirensis OFN S31T (97.75 %). Phylogenetic trees showed that strain J12GA03T firmly formed a distinct monophyletic branch in the clade with A . subflavus DQS3-9A1T and H . altamirensis DSM 45258T. The levels of DNA–DNA relatedness with A . subflavus DSM 45089T and H . altamirensis DSM 45258T were 39.7±3.9 % and 35.7±3.0 %, respectively. Combining the evidence from the polyphasic taxonomic study, strain J12GA03T represents a novel species of the genus Hoyosella , for which the name Hoyosella rhizosphaerae sp. nov. is proposed. The type strain is J12GA03T (=DSM 101985T=CGMCC 1.15478T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001416