@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001426, author = "Su, C.-C. and Deng, W.-L. and Jan, F.-J. and Chang, C.-J. and Huang, H. and Shih, H.-T. and Chen, J.", title = "Xylella taiwanensis sp. nov., causing pear leaf scorch disease", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "11", pages = "4766-4771", doi = "https://doi.org/10.1099/ijsem.0.001426", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001426", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "pear leaf scorch disease", keywords = "Xylella", keywords = "Xylella taiwanensis", keywords = "fastidious prokaryote", abstract = "A Gram-stain-negative, nutritionally fastidious bacterium (PLS229T) causing pear leaf scorch was identified in Taiwan and previously grouped into Xylella fastidiosa . Yet, significant variations between PLS229T and Xylella fastidiosa were noted. In this study, PLS229T was evaluated phenotypically and genotypically against representative strains of Xylella fastidiosa , including strains of the currently known subspecies of Xylella fastidiosa , Xylella fastidiosa subsp. multiplex and ‘ Xylella fastidiosa subsp. pauca ’. Because of the difficulty of in vitro culture characterization, emphases were made to utilize the available whole-genome sequence information. The average nucleotide identity (ANI) values, an alternative for DNA–DNA hybridization relatedness, between PLS229T and Xylella fastidiosa were 83.4–83.9 %, significantly lower than the bacterial species threshold of 95 %. In contrast, sequence similarity of 16S rRNA genes was greater than 98 %, higher than the 97 % threshold to justify if two bacterial strains belong to different species. The uniqueness of PLS229T was also evident by observing only about 87 % similarity in the sequence of the 16S-23S internal transcribed spacer (ITS) between PLS229T and strains of Xylella fastidiosa , discovering significant single nucleotide polymorphisms at 18 randomly selected housekeeping gene loci, observing a distinct fatty acid profile for PLS229T compared with Xylella fastidiosa , and PLS229T having different observable phenotypes, such as different susceptibility to antibiotics. A phylogenetic tree derived from 16S rRNA gene sequences showed a distinct PLS229T phyletic lineage positioning it between Xylella fastidiosa and members of the genus Xanthomonas . On the basis of these data, a novel species, Xylella taiwanensis sp. nov. is proposed. The type strain is PLS229T (=BCRC 80915T=JCM 31187T).", }