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f Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov.

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  • Authors: Ping-Hua Qu1​ ,  Ying Li1​,2​ ,  Nimaichand Salam3​ ,  Shou-Yi Chen4​ ,  Lan Liu3​ ,  Quan Gu5​ ,  Bao-Zhu Fang3​ ,  Min Xiao3​ ,  Mo Li1​ ,  Cha Chen1​ , Wen-Jun Li3​
    • VIEW AFFILIATIONS
    • 1​ 1​Department of Clinical Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, PR China 2​ 2​School of Laboratory Medicine, Tianjin Medical University, Tianjin 300070, PR China 3​ 3​State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China 4​ 4​Department of Microbiological Laboratory, Guangzhou Center for Diseases Control and Prevention, Guangzhou 510440, PR China 5​ 5​Department of Clinical Laboratory, Tangshan People’s Hospital, Tangshan 063000, PR China
  • Correspondence Cha Chen [email protected]
  • First Published Online: 01 November 2016, International Journal of Systematic and Evolutionary Microbiology 66: 4832-4838, doi: 10.1099/ijsem.0.001437
  • Subject: New taxa - Proteobacteria
  • Received:
  • Accepted:
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Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov., Page 1 of 1

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  • Five bacterial strains (SYSU YG23, SYSU 10HL1970, 10HP82-10, 10HL1938, 10HP457) isolated from water reservoirs of cooling systems were characterized using a polyphasic taxonomic approach. The isolates were Gram-stain-negative, strictly aerobic and non-motile. Growth was enhanced in the presence of l-cysteine. The major fatty acids (>5 %) for the five strains were C10 : 0, C16 : 0, C16 : 0 3-OH, C18 : 0 3-OH and C18 : 1 ω9c. Ubiquinone-8 was detected as the respiratory quinone while the polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, three unidentified phospholipids, two unidentified aminophospholipids and three unidentified glycolipids. The strains shared 16S rRNA gene sequence similarities of 99.0–99.2 % with Francisella guangzhouensis 08HL01032 but less than 95.2 % with other members of the family Francisellaceae . The phylogenetic dendrogram based on 16S rRNA gene sequences showed that these strains form a separate cluster along with Francisella guangzhouensis . This cluster was also confirmed from multilocus-sequence typing based on sequences of the mdhA, rpoB and sdhA genes. Matrix-assisted laser desorption ionization time-of-flight MS analyses of the strains along with closely and distantly related Francisella strains also showed a distinct cluster for these strains. Based on the findings from the polyphasic taxonomy studies, the strains were considered to represent two novel species of a new genus for which the names Allofrancisella inopinata gen. nov., sp. nov. (type strain SYSU YG23=KCTC 42968=DSM 101834) and Allofrancisella frigidaquae sp. nov. (type strain SYSU 10HL1970=KCTC 42969=DSM 101835) are proposed. In addition, Francisella guangzhouensis Qu et al. 2013 is proposed to be transferred to this new genus as Allofrancisella guangzhouensis comb. nov.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, mdhA, rpoB and sdhA gene sequences are given in Table S1.

  • Six supplementary figures and two supplementary tables are available with the online Supplementary Material.

  • Abbreviation:
    MALDI-TOF matrix-assisted laser desorption ionization time-of-flight

© 2016 IUMS | Published by the Microbiology Society

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