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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 11

sp. nov., isolated from mixed deciduous forest soil Free

Abstract

A novel filamentous bacterial strain, A-T 5400, which developed subglobose sporangia at the end of sporangiophores on substrate mycelia, was isolated from mixed deciduous forest soil collected in Thailand. The taxonomic position of this micro-organism was described using a polyphasic approach. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 5400 belonged to the genus and was most closely related to ‘' NEAU-M9 (98.82 % 16S rRNA gene sequence similarity) and NBRC 13878 (98.75 %). The DNA–DNA relatedness values that distinguished the novel strain from the closest species were below 70 %. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars were ribose, galactose, glucose and xylose. The predominant menaquinone was MK-9(H). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were unsaturated fatty acids C, branched fatty acids iso-C and iso-C. The G+C content of the genomic DNA was 71 mol%. Following evidence from phenotypic, chemotaxonomic and genotypic studies, the new isolate is proposed to represent a novel species of the genus named sp. nov. The type strain is A-T 5400 (=BCC 42734=TBRC 5832=NBRC 109645).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinobacteria , Actinoplanes subglobosus and Thailand
© 2016 IUMS
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2016-11-01
2024-04-19
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Actinoplanes subglobosus sp. nov., isolated from mixed deciduous forest soil
Int J Syst Evol Microbiol 66, 4850 (2016); https://doi.org/10.1099/ijsem.0.001440
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