Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States
Pritt, Bobbi S. and Respicio-Kingry, Laurel B. and Sloan, Lynne M. and Schriefer, Martin E. and Replogle, Adam J. and Bjork, Jenna and Liu, Gongping and Kingry, Luke C. and Mead, Paul S. and Neitzel, David F. and Schiffman, Elizabeth and Hoang Johnson, Diep K. and Davis, Jeffrey P. and Paskewitz, Susan M. and Boxrud, David and Deedon, Alecia and Lee, Xia and Miller, Tracy K. and Feist, Michelle A. and Steward, Christopher R. and Theel, Elitza S. and Patel, Robin and Irish, Cole L. and Petersen, Jeannine M.,, 66, 4878-4880 (2016),
doi = https://doi.org/10.1099/ijsem.0.001445,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Lyme borreliosis (LB) is a multisystem disease caused by spirochetes in the Borrelia burgdorferi sensu lato (Bbsl) genospecies complex. We previously described a novel Bbsl genospecies (type strain MN14-1420T) that causes LB among patients with exposures to ticks in the upper midwestern USA. Patients infected with the novel Bbsl genospecies demonstrated higher levels of spirochetemia and somewhat differing clinical symptoms as compared with those infected with other Bbsl genospecies. The organism was detected from human specimens using PCR, microscopy, serology and culture. The taxonomic status was determined using an eight-housekeeping-gene (uvrA, rplB, recG, pyrG, pepX, clpX, clpA and nifS) multi-locus sequence analysis (MLSA) and comparison of 16S rRNA gene, flaB, rrf–rrl, ospC and oppA2 nucleotide sequences. Using a system threshold of 98.3 % similarity for delineation of Bbsl genospecies by MLSA, we demonstrated that the novel species is a member of the Bbsl genospecies complex, most closely related to B. burgdorferi sensu stricto (94.7–94.9 % similarity). This same species was identified in Ixodes scapularis ticks collected in Minnesota and Wisconsin. This novel species, Borrelia mayonii sp. nov, is formally described here. The type strain, MN14-1420, is available through the Deutsche Sammlung von Mikroorganismen und Zelkulturen GmbH (DSM 102811) and the American Type Culture Collection (ATCC BAA-2743).,
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