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f Reclassification of strains MAFF 303099 and R7A into Mesorhizobium japonicum sp. nov.

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  • Authors: Pilar Martínez-Hidalgo1​ ,  Martha Helena Ramírez-Bahena2​,3​ ,  José David Flores-Félix1​ ,  José M. Igual2​,3​ ,  Juan Sanjuán4​ ,  Milagros León-Barrios5​ ,  Alvaro Peix2​,3​ , Encarna Velázquez1​,3​
    • VIEW AFFILIATIONS
    • 1​ 1​Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain 2​ 2​Desarrollo Sostenible de Sistemas Agroforestales y Ganaderos, IRNASA-CSIC, Salamanca, Spain 3​ 3​Unidad Asociada Universidad de Salamanca-CSIC ‘Interacciones Planta-Microorganismo’, Salamanca, Spain 4​ 4​Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidin, CSIC, Granada, Spain 5​ 5​Departamento de Bioquímica, Microbiología, Biología Celular y Genética, Universidad de La Laguna, Tenerife, Spain
  • Correspondence Encarna Velázquez [email protected]
  • First Published Online: 01 December 2016, International Journal of Systematic and Evolutionary Microbiology 66: 4936-4941, doi: 10.1099/ijsem.0.001448
  • Subject: New taxa - Proteobacteria
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Reclassification of strains MAFF 303099 and R7A into Mesorhizobium japonicum sp. nov., Page 1 of 1

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  • In this work we revise the taxonomic status of the Lotus-nodulating strains MAFF 303099 and R7A isolated in Japan and New Zealand, respectively. Their 16S rRNA gene sequences are identical and show 98.0, 99.7, 99.8 and 99.9 % similarity values with respect to Mesorhizobium loti NZP 2213, M. jarvisii ATCC 33669, M. huakuii USDA 4779 (=CCBAU 2609) and M. erdmanii USDA 3471, respectively. The analysis of recA and glnII gene sequeces showed that M. jarvisii ATCC 33669 and M. huakuii USDA 4779 (=CCBAU 2609) are the most closely related strains to MAFF 303099 and R7A, with similarity values suggesting that these two strains belong to a different species for which MAFF 303099 is selected as the type strain. The DNA–DNA relatedness values between strain MAFF 303099 and its closest phylogenetic relatives ranged from 53 to 60 % in average. Strains MAFF 303099 and R7A presented slight differences in the proportions of C18 : 1ω7c 11-methyl and C19 : 0 cyclo ω8c fatty acids with respect to M. jarvisii ATCC 33669 and M. huakuii USDA 4779, and also in several phenotypic characteristics. Therefore, we propose the reclassification of these two strains into a novel species named Mesorhizobium japonicum sp. nov., with the type strain being MAFF 303099 (=LMG 29417=CECT 9101).

  • Three supplementary figures and one supplementary table are available with the online Supplementary Material.

  • Abbreviation:
    ANI average nucleotide identity

© 2016 IUMS | Published by the Microbiology Society

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2016-12-01
2019-02-19
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