@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001451, author = "Xia, Xian and Wu, Shijuan and Han, Yushan and Liao, Shuijiao and Wang, Gejiao", title = "Pelobium manganitolerans gen. nov., sp. nov., isolated from sludge of a manganese mine", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "12", pages = "4954-4959", doi = "https://doi.org/10.1099/ijsem.0.001451", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001451", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "manganese mine", keywords = "sludge", keywords = "Sphingobacteriaceae", abstract = "A Gram-staining-negative, rod-shaped, motile by gliding and facultative anaerobic bacterial strain, YS-25T, was isolated from a sludge of a manganese mine. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YS-25T formed a single branch within the strains of the family Sphingobacteriaceae and showed low similarities to Pedobacter arcticus CCTCC AB 2010223T (91.7 %), ‘ Pedobacter zeaxanthinifaciens ’ TDMA-5 (91.5 %), Pedobacter terricola DS-45T (90.9 %), Pedobacter ureilyticus THG-T11T (90.9 %), Pseudopedobacter beijingensis MCCC 1A01299T (90.8 %) and Pedobacter heparinus CCTCC AB 209030T (88.5 %). Strain YS-25T had some unique physiological and biochemical characteristics: facultative anaerobic, able to hydrolyse Tween 40, positive for cystine arylamidase and negative for mannose assimilation and β-galactosidase. The polar lipids of strain YS-25T were phosphatidylethanolamine, aminolipid, two unknown lipids and two glycolipids. The presence of glycolipids and absence of sphingolipid were different from the Pedobacter and Pseudopedobacter strains. The major fatty acids (>5 %) were iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), unknown ECL 13.565, iso-C17 : 0 3-OH, iso-C17 : 1 ω9c and anteiso-C15 : 0. The genomic DNA G+C content was 42.0 mol%, and the only respiratory quinone was menaquinone 7. On the basis of polyphasic taxonomic analyses, strain YS-25T is considered to represent a novel genus and species, for which the name Pelobium manganitolerans gen. nov., sp. nov. is proposed. The type strain is YS-25T (=KCTC 52203T=CCTCC AB 2016051T).", }