%0 Journal Article %A Zhang, Binglin %A Tang, Shukun %A Chen, Ximing %A Zhang, Ling %A Zhang, Gaoseng %A Zhang, Wei %A Liu, Guangxiu %A Chen, Tuo %A Li, Shiweng %A Dyson, Paul %T Streptomyces lacrimifluminis sp. nov., a novel actinobacterium that produces antibacterial compounds, isolated from soil %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 12 %P 4981-4986 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001456 %K taxonomy %K actinobacterium %K Stretomyces sp. nov. %I Microbiology Society, %X A novel actinobacterial strain, designated Z1027T, was isolated from a soil sample collected near the Tuotuo River, Qinghai–Tibet Plateau (China). The strain exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus . The taxonomic position of strain Z1027T was determined using a polyphasic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree, together with Streptomyces turgidiscabies ATCC 700248T (99.19 % similarity), Streptomyces graminilatus JL-6T (98.84 %) and Streptomyces reticuliscabiei CFBP 4531T (98.36 %). The genomic DNA G+C content of strain Z1027T was 74±1 mol%. The DNA–DNA relatedness values between strain Z1027T and Streptomyces turgidiscabies ATCC 700248T and Streptomyces reticuliscabiei CFBP 4531T were 38.5±0.4 and 26.2±1.2 %, respectively, both of them significantly lower than 70 %. Chemotaxonomic data revealed that strain Z1027T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, ll-diaminopimelic acid as the diagnostic diamino acid and galactose as a whole-cell sugar. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatydilinositol and seven other unknown polar lipids were detected; iso-C16 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0 were the major fatty acids. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1027T (=CGMCC 4.7272T=JCM 31054T) should be classified as the type strain of a novel species of the genus Streptomyces , Streptomyces lacrimifluminis sp. nov. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001456