RT Journal Article SR Electronic(1) A1 Zhang, Jun A1 Ma, Xiao-Tong A1 Gao, Jun-Sheng A1 Zhao, Juan-Juan A1 Yin, Hua-Qun A1 Zhang, Cai-Wen A1 Zhang, Rui-Jie A1 Zhang, Xiao-XiaYR 2016 T1 Paenibacillusoryzae sp. nov., isolated from rice roots JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5000 OP 5004 DO https://doi.org/10.1099/ijsem.0.001459 PB Microbiology Society, SN 1466-5034, AB A novel endophytic bacterium, strain 1DrF-4T, isolated from rice roots, was characterized on the basis of its phenotypic characteristics and genotypic information. The novel strain was Gram-positive-staining, endospore-forming, facultatively anaerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1DrF-4T formed a monophyletic clade within the genus Paenibacillus . The most phylogenetically related species was Paenibacillus pinesoli KACC 17472T, with which strain 1DrF-4T showed 16S rRNA gene sequence similarity of 95.2 %. 16S rRNA gene sequence similarities with type strains of other species of the genus Paenibacillus were less than 95 %. The predominant cellular fatty acids were anteiso-C15 : 0 (61.1 %) and C16 : 0 (11.1 %), which is one of the characteristic traits of the genus Paenibacillus . The quinone system contained exclusively menaquinone MK-7. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and an unknown phospholipid. The DNA G+C content was 50.16 mol%, which was within the range reported for species of the genus Paenibacillus . Characterization by genotypic, chemotaxonomic and phenotypic analysis indicated that strain 1DrF-4T (=ACCC 19927T=JCM 30486T) represents a novel species of the genus Paenibacillus , for which the name Paenibacillus oryzae sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001459