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f Streptomyces tremellae sp. nov., isolated from a culture of the mushroom Tremella fuciformis

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Streptomyces tremellae sp. nov., isolated from a culture of the mushroom Tremella fuciformis, Page 1 of 1

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  • A novel actinomycete strain, designated Js-1, was isolated from Tremella fuciformis collected from Gutian, Fujian Province, in southeastern China. The taxonomic status of this strain was determined by a polyphasic approach, which demonstrated that the novel strain was a member of the genus Streptomyces . The cell walls of this strain were found to contain ll-diaminopimelic acid, muramic acid and glycine. An analysis of whole-cell hydrolysates revealed that no characteristic sugar was present. The key identified menaquinones were MK-9 (H6) and MK-9 (H8), while the diagnostic polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmethylethanolamine and phosphatidylglycerol. The main cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, C16 : 0 and iso-C16 : 0. An analysis of an almost complete 16S rRNA gene sequence showed that the strain shared the highest levels of sequence similarity with Streptomyces sannanensis KC-7038 (97.87 %), Streptomyces hebeiensis YIM 001 (97.84 %), Streptomyces pathocidini NBRC 13812 (97.80 %), Streptomyces cocklensis BK168 (97.25 %), Streptomyces coerulescens NBRC 12758 (97.12 %), Streptomyces aurantiogriseus NBRC 12842 (97.06 %) and Streptomyces rimosus subsp. rimosus ATCC 10970 (97.04 %). The DNA G+C content of the genomic DNA of strain Js-1 was 70.1 mol%. Furthermore, DNA–DNA hybridization tests revealed that the relatedness values between strain Js-1 and the most closely related species ranged from 15.10 to 47.20 %. Based on its phenotypic and genotypic characteristics, strain Js-1 (=CCTCC M 2011365=JCM 30846) is considered to represent a novel species within the genus Streptomyces , which we classified as Streptomyces tremellae sp. nov.

  • †

    These authors contributed equally to this work

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Js-1 is JN873308.

  • Four supplementary figures and two supplementary tables are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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2016-12-01
2019-02-19
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