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- Volume 66, Issue 12
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f Pseudoxanthomonas helianthi sp. nov., isolated from roots of Jerusalem artichoke (Helianthus tuberosus)
- Authors: Chokchai Kittiwongwattana1,2 , Chitti Thawai1,2
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1 1Department of Biology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 2Actinobacterial Research Unit, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
- Correspondence Chokchai Kittiwongwattana [email protected]
- First Published Online: 01 December 2016, International Journal of Systematic and Evolutionary Microbiology 66: 5034-5038, doi: 10.1099/ijsem.0.001465
- Subject: New taxa - Proteobacteria
- Received:
- Accepted:
- Cover date:




Pseudoxanthomonas helianthi sp. nov., isolated from roots of Jerusalem artichoke (Helianthus tuberosus), Page 1 of 1
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A bacterium designated as strain roo10T was isolated from roots of Jerusalem artichoke (Helianthus tuberosus). Cells were Gram-stain-negative and non-motile rods. The phylogenetic analysis of the 16S rRNA gene indicated that it represented a member of the genus Pseudoxanthomonas , and its close relatives included Pseudoxanthomonas kalamensis JA40T (97.8 % 16S rRNA gene sequence similarity), Pseudoxanthomonas sangjuensis 5GH38-5T (97.7 %) and Pseudoxanthomonas daejeonensis TR6-08T (97.1 %). Growth of roo10T occurred at pH 7–9. The temperature for growth ranged from 20 to 37 °C. Tolerance to NaCl was observed from 0.005 to 5 % (w/v) concentration. Predominant fatty acids were iso-C15 : 0 (23.5 %), iso-C16 : 0 (18.9 %) and anteiso-C15 : 0 (11.5 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl-N-methylethanolamine were the major polar lipids. The predominant quinone was ubiquinone 8 (Q-8). The DNA G+C content was 65.7 mol% [from melting temperature (Tm )]. Comparison of phenotypic and chemotaxonomic characteristics indicated that roo10T was distinguishable from its close relatives. Additionally, the DNA–DNA relatedness levels between roo10T and P. kalamensis DSM 18571T (22±0.5 %), P. sangjuensis 5GH38-5T (21±0.2 %) and P. daejeonensis DSM 17801T (3±1 %) were lower than 70 %. These results indicated that roo10T represented a novel species of the genus Pseudoxanthomonas , for which the name Pseudoxanthomonas helianthi sp. nov. is proposed. The type strain is roo10T (=BCC 70700T=NBRC 110414T).
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The GenBank/EMBL/DDBJ accession number for the partial 16S rRNA gene sequence of strain roo10T is AB905612.
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Four supplementary figures and one supplementary table are available with the online Supplementary Material.
- Keyword(s): Pseudoxanthomonas, Endophytes, Helianthus tuberosus
© 2016 IUMS | Published by the Microbiology Society
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