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Volume 66, Issue 12

sp. nov., isolated from roots of Jerusalem artichoke () Free

Abstract

A bacterium designated as strain roo10 was isolated from roots of Jerusalem artichoke (). Cells were Gram-stain-negative and non-motile rods. The phylogenetic analysis of the 16S rRNA gene indicated that it represented a member of the genus , and its close relatives included JA40 (97.8 % 16S rRNA gene sequence similarity), 5GH38-5 (97.7 %) and TR6-08 (97.1 %). Growth of roo10 occurred at pH 7–9. The temperature for growth ranged from 20 to 37 °C. Tolerance to NaCl was observed from 0.005 to 5 % (w/v) concentration. Predominant fatty acids were iso-C (23.5 %), iso-C (18.9 %) and anteiso-C (11.5 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl--methylethanolamine were the major polar lipids. The predominant quinone was ubiquinone 8 (Q-8). The DNA G+C content was 65.7 mol% [from melting temperature ()]. Comparison of phenotypic and chemotaxonomic characteristics indicated that roo10 was distinguishable from its close relatives. Additionally, the DNA–DNA relatedness levels between roo10 and DSM 18571 (22±0.5 %), 5GH38-5 (21±0.2 %) and DSM 17801 (3±1 %) were lower than 70 %. These results indicated that roo10 represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is roo10 (=BCC 70700=NBRC 110414).

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Keyword(s): Endophytes , Helianthus tuberosus and Pseudoxanthomonas
© 2016 IUMS
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2016-12-01
2024-04-24
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Pseudoxanthomonas helianthi sp. nov., isolated from roots of Jerusalem artichoke (Helianthus tuberosus)
Int J Syst Evol Microbiol 66, 5034 (2016); https://doi.org/10.1099/ijsem.0.001465
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