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f Pseudoxanthomonas helianthi sp. nov., isolated from roots of Jerusalem artichoke (Helianthus tuberosus)

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  • Authors: Chokchai Kittiwongwattana1​,2​ , Chitti Thawai1​,2​
    • VIEW AFFILIATIONS
    • 1​ 1​Department of Biology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2​ 2​Actinobacterial Research Unit, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
  • Correspondence Chokchai Kittiwongwattana [email protected]
  • First Published Online: 01 December 2016, International Journal of Systematic and Evolutionary Microbiology 66: 5034-5038, doi: 10.1099/ijsem.0.001465
  • Subject: New taxa - Proteobacteria
  • Received:
  • Accepted:
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Pseudoxanthomonas helianthi sp. nov., isolated from roots of Jerusalem artichoke (Helianthus tuberosus), Page 1 of 1

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  • A bacterium designated as strain roo10 was isolated from roots of Jerusalem artichoke (Helianthus tuberosus). Cells were Gram-stain-negative and non-motile rods. The phylogenetic analysis of the 16S rRNA gene indicated that it represented a member of the genus Pseudoxanthomonas , and its close relatives included Pseudoxanthomonas kalamensis JA40 (97.8 % 16S rRNA gene sequence similarity), Pseudoxanthomonas sangjuensis 5GH38-5 (97.7 %) and Pseudoxanthomonas daejeonensis TR6-08 (97.1 %). Growth of roo10 occurred at pH 7–9. The temperature for growth ranged from 20 to 37 °C. Tolerance to NaCl was observed from 0.005 to 5 % (w/v) concentration. Predominant fatty acids were iso-C15 : 0 (23.5 %), iso-C16 : 0 (18.9 %) and anteiso-C15 : 0 (11.5 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl-N-methylethanolamine were the major polar lipids. The predominant quinone was ubiquinone 8 (Q-8). The DNA G+C content was 65.7 mol% [from melting temperature (Tm )]. Comparison of phenotypic and chemotaxonomic characteristics indicated that roo10 was distinguishable from its close relatives. Additionally, the DNA–DNA relatedness levels between roo10 and P. kalamensis DSM 18571 (22±0.5 %), P. sangjuensis 5GH38-5 (21±0.2 %) and P. daejeonensis DSM 17801 (3±1 %) were lower than 70 %. These results indicated that roo10 represented a novel species of the genus Pseudoxanthomonas , for which the name Pseudoxanthomonas helianthi sp. nov. is proposed. The type strain is roo10 (=BCC 70700=NBRC 110414).

  • The GenBank/EMBL/DDBJ accession number for the partial 16S rRNA gene sequence of strain roo10 is AB905612.

  • Four supplementary figures and one supplementary table are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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