1887

Abstract

A novel actinomycete, strain TRM 41495, was isolated from lake sediment of Xiaoerkule lake in Xinjiang province, China, and was examined using a polyphasic approach. Strain TRM 41495 was aerobic, Gram-stain-positive, growing best on ISP medium 7 with abundant white aerial mycelium formed and brown soluble pigments produced. The optimum NaCl concentration for growth was 3.0 % (w/v), and the optimum temperature for growth was 37 °C. On the basis of 16S rRNA gene sequence analysis, strain TRM 41495 was shown to belong to the genus with a sequence similarity of 97.50 % with the most closely related species, . The organism had chemical and morphological features typical of the genus . The cell wall of strain TRM 41495 contained -diaminopimelic acid, and galactose, arabinose, ribose and mannose were the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and an unknown phosphoglycolipid. The predominant menaquinones were MK-9(H) and MK-9(H). The major fatty acids were iso-C, C, Cω6, anteiso-C and iso-CH. The G+C content of the DNA was 72.9 mol%. However, DNA–DNA hybridization studies between strain TRM 41495 and showed only 43.9 % relatedness. Whole-cell sugars, menaquinones and fatty acids differentiate strain TRM 41495 from . On the basis of the polyphasic evidence, strain TRM 41495 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 41495 (=CCTCC AA 2015038=KCTC 39727).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001487
2016-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5145.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001487&mimeType=html&fmt=ahah

References

  1. Al-Zarban S. S., Al-Musallam A. A., Abbas I., Stackebrandt E., Kroppenstedt R. M. 2002; Saccharomonospora halophila sp. nov., a novel halophilic actinomycete isolated from marsh soil in Kuwait. Int J Syst Evol Microbiol 52:555–558 [CrossRef]
    [Google Scholar]
  2. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
    [Google Scholar]
  3. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics , pp. 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Filipski A., Murillo O., Freydenzon A., Tamura K., Kumar S. 2014; Prospects for building large timetrees using molecular data with incomplete gene coverage among species. Mol Biol Evol 31:2542–2550 [View Article]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  8. Greiner-Mai E., Korn-Wendisch F., Kutzner H. J. 1988; Taxonomic revision of the genus Saccharomonospora and description of Saccharomonospora glauca sp. nov. Int J Syst Bacteriol 38:398–405 [View Article]
    [Google Scholar]
  9. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [View Article][PubMed]
    [Google Scholar]
  10. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
    [Google Scholar]
  11. He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J. 2005; Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939–1944 [View Article][PubMed]
    [Google Scholar]
  12. Jin X., Xu L. H., Mao P. H., Hseu T. H., Jiang C. L. 1998; Description of Saccharomonospora xinjiangensis sp. nov. based on chemical and molecular classification. Int J Syst Bacteriol 48:1095–1099 [View Article][PubMed]
    [Google Scholar]
  13. Kelly K. L. 1964 Inter-Society Color Council-National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  15. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
    [Google Scholar]
  16. Li W. J., Tang S. K., Stackebrandt E., Kroppenstedt R. M., Schumann P., Xu L. H., Jiang C. L. 2003; Saccharomonospora paurometabolica sp. nov., a moderately halophilic actinomycete isolated from soil in China. Int J Syst Evol Microbiol 53:1591–1594 [CrossRef]
    [Google Scholar]
  17. Liu Z., Li Y., Zheng L. Q., Huang Y. J., Li W. J. 2010; Saccharomonospora marina sp. nov., isolated from an ocean sediment of the East China Sea. Int J Syst Evol Microbiol 60:1854–1857 [View Article][PubMed]
    [Google Scholar]
  18. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  19. Nonomura H., Ohara Y. 1971; Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes. J Ferment Technol 49:895–903
    [Google Scholar]
  20. Runmao H. 1987; Saccharomonospora azurea sp. nov., a new species from soil. Int J Syst Bacteriol 37:60–61 [View Article]
    [Google Scholar]
  21. Runmao H. U., Cheng L. 1988; Saccharomonospora cyanea sp. nov. Int J Syst Bacteriol 38:444–446 [View Article]
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  23. Schuurmans D. M., Olson B. H., Clemente A. C. L. S. 1956; Production and isolation of thermoviridin, an antibiotic produced by Thermoactinomyces viridis n. sp. Appl Microbiol 4:61–66[PubMed]
    [Google Scholar]
  24. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  25. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  26. Syed D. G., Tang S. K., Cai M., Zhi X. Y., Agasar D., Lee J. C., Kim C. J., Jiang C. L., Xu L. H. et al. 2008; Saccharomonospora saliphila sp. nov., a halophilic actinomycete from an Indian soil. Int J Syst Evol Microbiol 58:570–573 [View Article][PubMed]
    [Google Scholar]
  27. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  28. Veyisoglu A., Sazak A., Cetin D., Guven K., Sahin N. 2013; Saccharomonospora amisosensis sp. nov. isolated from deep marine sediment. Int J Syst Evol Microbiol 63:3782–3786 [View Article][PubMed]
    [Google Scholar]
  29. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 , pp. 2452–2492 Baltimore, MD:: Williams & Wilkins;
    [Google Scholar]
  30. Zhang D. F., Chen W., He J., Zhang X. M., Xiong Z. J., Sahu M. K., Sivakumar K., Li W. J. 2013; Saccharomonospora oceani sp. nov. isolated from marine sediments in Little Andaman, India. Antonie Van Leeuwenhoek 103:1377–1384 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001487
Loading
/content/journal/ijsem/10.1099/ijsem.0.001487
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error