RT Journal Article SR Electronic(1) A1 Li, Dong A1 Chen, Zheng-Jun A1 Luo, Xiao-Xia A1 Xia, Zhan-Feng A1 Wan, Chuan-Xing A1 Zhang, Li-LiYR 2016 T1 Saccharomonospora xiaoerkulensis sp. nov., isolated from lake sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5145 OP 5149 DO https://doi.org/10.1099/ijsem.0.001487 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, strain TRM 41495T, was isolated from lake sediment of Xiaoerkule lake in Xinjiang province, China, and was examined using a polyphasic approach. Strain TRM 41495T was aerobic, Gram-stain-positive, growing best on ISP medium 7 with abundant white aerial mycelium formed and brown soluble pigments produced. The optimum NaCl concentration for growth was 3.0 % (w/v), and the optimum temperature for growth was 37 °C. On the basis of 16S rRNA gene sequence analysis, strain TRM 41495T was shown to belong to the genus Saccharomonospora with a sequence similarity of 97.50 % with the most closely related species, Saccharomonospora xinjiangensis . The organism had chemical and morphological features typical of the genus Saccharomonospora . The cell wall of strain TRM 41495T contained meso-diaminopimelic acid, and galactose, arabinose, ribose and mannose were the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and an unknown phosphoglycolipid. The predominant menaquinones were MK-9(H4) and MK-9(H2). The major fatty acids were iso-C16 : 0, C16 : 0, C17 : 1ω6c, anteiso-C17 : 0 and iso-C16 : 1H. The G+C content of the DNA was 72.9 mol%. However, DNA–DNA hybridization studies between strain TRM 41495T and Saccharomonospora xinjiangensis showed only 43.9 % relatedness. Whole-cell sugars, menaquinones and fatty acids differentiate strain TRM 41495T from Saccharomonospora xinjiangensis . On the basis of the polyphasic evidence, strain TRM 41495T represents a novel species of the genus Saccharomonospora , for which the name Saccharomonospora xiaoerkulensis sp. nov. is proposed. The type strain is TRM 41495T (=CCTCC AA 2015038T=KCTC 39727T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001487