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f Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp. nov., Kazachstania serrabonitensis sp. nov. and Kazachstania australis sp. nov. Reassignment of Candida humilis to Kazachstania humilis f.a. comb. nov. and Candida pseudohumilis to Kazachstania pseudohumilis f.a. comb. nov.
- Authors: Noémie Jacques1 , Véronique Sarilar1 , Charlotte Urien2 , Mariana R. Lopes3 , Camila G. Morais3 , Ana Paula T. Uetanabaro4 , Colin R. Tinsley1 , Carlos A. Rosa3 , Delphine Sicard5 , Serge Casaregola1
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1 1Micalis Institute, INRA, AgroParisTech, CIRM-Levures, Université Paris-Saclay, 78350 Jouy-en-Josas, France 2 2Génétique Quantitative et Evolution-Le Moulon, INRA, Université Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, 91190 Gif-sur-Yvette, France 3 3Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil 4 4Departamento de Ciências Biológicas e Agroindústria, Universidade Estadual Santa Cruz, Ilhéus, BA 45662-900, Brazil 5 5Sciences pour l’œnologie, INRA, Supagro, 34060 Montpellier, France
- Correspondence Serge Casaregola [email protected]
- First Published Online: 01 December 2016, International Journal of Systematic and Evolutionary Microbiology 66: 5192-5200, doi: 10.1099/ijsem.0.001495
- Subject: New taxa - Eukaryotic micro-organisms
- Received:
- Accepted:
- Cover date:




Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp. nov., Kazachstania serrabonitensis sp. nov. and Kazachstania australis sp. nov. Reassignment of Candida humilis to Kazachstania humilis f.a. comb. nov. and Candida pseudohumilis to Kazachstania pseudohumilis f.a. comb. nov., Page 1 of 1
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Five ascosporogenous yeast strains related to the genus Kazachstania were isolated. Two strains (CLIB 1764T and CLIB 1780) were isolated from French sourdoughs; three others (UFMG-CM-Y273T, UFMG-CM-Y451 and UFMG-CM-Y452) were from rotting wood in Brazil. The sequences of the French and Brazilian strains differed by one and three substitutions, respectively, in the D1/D2 large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS). The D1/D2 LSU rRNA sequence of these strains differed by 0.5 and 0.7 % from Kazachstania exigua, but their ITS sequences diverged by 8.1 and 8.3 %, respectively, from that of the closest described species Kazachstania barnettii. Analysis of protein coding sequences of RPB1, RPB2 and EF-1α distinguished the French from the Brazilian strains, with respectively 3.3, 6 and 11.7 % substitutions. Two novel species are described to accommodate these newly isolated strains: Kazachstania saulgeensis sp. nov. (type strain CLIB 1764T=CBS 14374T) and Kazachstania serrabonitensis sp. nov. (type strain UFMG-CM-Y273T=CLIB 1783T=CBS 14236T). Further analysis of culture collections revealed a strain previously assigned to the K. exigua species, but having 3.8 % difference (22 substitutions and 2 indels) in its ITS with respect to K. exigua. Hence, we describe a new taxon, Kazachstania australis sp. nov. (type strain CLIB 162T=CBS 2141T), to accommodate this strain. Finally, Candida humilis and Candida pseudohumilis are reassigned to the genus Kazachstania as new combinations. On the basis of sequence analysis, we also propose that Candida milleri and Kazachstania humilis comb. nov. are conspecific.
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The GenBank/EMBL/DDBJ accession numbers for the sequences determined in this study are KF582608–KF582610, LT158238, LT158239, LT158241, LT158244, LT158246–LT158251, LT158253, LT158254, LT158258, LT158259, LT158261, LT158262, LT158264–LT158271, LT158273–LT158276, LT158278, LT158280, LT158282–LT158284 and LT594998–LT595004, see Table S1 (available in the online Supplementary Material) for details. The Mycobank (http://www.mycobank.org) accession numbers for Kazachstania saulgeensis sp. nov., Kazachstania serrabonitensis sp. nov., Kazachstania australis sp. nov., Kazachstania humilis f.a. comb. nov. and Kazachstania pseudohumilis f.a. comb. nov. are, respectively, MB 815524, MB 815525, MB 815523, MB 815526 and MB 815527.
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Two supplementary tables are available with the online Supplementary Material.
- Keyword(s): South Africa, Brazil, soil, France, rotting wood, Kazachstania saulgeensis sp. nov.
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Abbreviations: EF-1α translation elongation factor-1α ITS internal transcribed spacer LSU large subunit
© 2016 IUMS | Published by the Microbiology Society
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