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f Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower

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  • Authors: Zheng-Fei Yan1​ ,  Pei Lin1​ ,  Yu-Shuai Wang1​ ,  Wei Gao1​ ,  Chang-Tian Li2​ ,  Moo-Chang Kook3​ , Tae-Hoo Yi1​
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    • 1​ 1​Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do, 46-701, Republic of Korea 2​ 2​Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China 3​ 3​Department of Marine Biotechnology, Anyang University, Incheon 417833, Republic of Korea
  • Correspondence Tae-Hoo Yi [email protected]
  • First Published Online: 01 December 2016, International Journal of Systematic and Evolutionary Microbiology 66: 5218-5222, doi: 10.1099/ijsem.0.001498
  • Subject: New taxa - Bacteroidetes
  • Received:
  • Accepted:
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Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower, Page 1 of 1

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  • A Gram-stain-negative, aerobic, non-motile, long rods with no flagellum strain, designated THG-YS3.2.1, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10−40 °C (optimum 28 °C), at pH 6.0−8.0 (optimum pH 7.0) and with 0−1.0 % NaCl (optimum 1.0 %). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C12 : 0, iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1 G, anteiso-C15 : 1 A, C15 : 0 2-OH, C16 : 0, iso-C16 : 0, C16 : 0 3-OH, iso-C16 : 0 3-OH, iso-C16 : 1 G, C17 : 0 2-OH, iso-C17 : 0 3-OH, C17 : 1ω6c, C18 : 3ω6c (6, 9, 12), C18 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major polar lipids were phosphatidylmethylethanolamine), phosphatidylethanolamine, five unidentified aminolipids and three unidentified lipids. The DNA G+C content of strain THG-YS3.2.1 was 45.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.1 were Niastella populi KCTC 22560 (98.7 % 16S rRNA gene sequence similarity), Niastella gongjuensis KACC 17399 (96.9 %), Niastella vici KCTC 42474 (96.2 %), Niastella yeongjuensis KACC 11466 (95.5 %) and Niastella koreensis KACC 11465 (95.1 %). DNA–DNA hybridization values between strain THG-YS3.2.1 and N. populi KCTC 22560, N. gongjuensis KACC 17399, N . vici KCTC 42474, N. yeongjuensis KACC 11466 and N. koreensis KACC 11465were 55.8±1.0, 39.5±0.5, 35.2±0.1, 17.6±0.3 and 12.5±1.2 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA−DNA hybridization data, strain THG-YS3.2.1 represents a novel species of the genus Niastella , for which the name Niastella hibisci sp. nov. is proposed. The type strain is THG-YS3.2.1 (=KCTC 52084=CCTCC AB 2015356).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain THG-YS3.2.1 is KT962169.

  • A supplementary table and four supplementary figures are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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