1887
  1. Home
  2. Publications
  3. International Journal of Systematic and Evolutionary Microbiology
  4. Volume 66, Issue 12
  5. Article

f Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower

HTML
57.58 Kb
PDF
162.97 Kb
  • Authors: Zheng-Fei Yan1​ ,  Pei Lin1​ ,  Yu-Shuai Wang1​ ,  Wei Gao1​ ,  Chang-Tian Li2​ ,  Moo-Chang Kook3​ , Tae-Hoo Yi1​
    • VIEW AFFILIATIONS
    • 1​ 1​Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do, 46-701, Republic of Korea 2​ 2​Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China 3​ 3​Department of Marine Biotechnology, Anyang University, Incheon 417833, Republic of Korea
  • Correspondence Tae-Hoo Yi [email protected]
  • First Published Online: 01 December 2016, International Journal of Systematic and Evolutionary Microbiology 66: 5218-5222, doi: 10.1099/ijsem.0.001498
  • Subject: New taxa - Bacteroidetes
  • Received:
  • Accepted:
  • Cover date:
Preview this:
Free Preview thumbnail Magnify
Zoom in
Zoomout

Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower, Page 1 of 1

| /docserver/preview/fulltext/ijsem/66/12/5218_ijsem001498-1.gif

  • A Gram-stain-negative, aerobic, non-motile, long rods with no flagellum strain, designated THG-YS3.2.1, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10−40 °C (optimum 28 °C), at pH 6.0−8.0 (optimum pH 7.0) and with 0−1.0 % NaCl (optimum 1.0 %). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C12 : 0, iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1 G, anteiso-C15 : 1 A, C15 : 0 2-OH, C16 : 0, iso-C16 : 0, C16 : 0 3-OH, iso-C16 : 0 3-OH, iso-C16 : 1 G, C17 : 0 2-OH, iso-C17 : 0 3-OH, C17 : 1ω6c, C18 : 3ω6c (6, 9, 12), C18 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major polar lipids were phosphatidylmethylethanolamine), phosphatidylethanolamine, five unidentified aminolipids and three unidentified lipids. The DNA G+C content of strain THG-YS3.2.1 was 45.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.1 were Niastella populi KCTC 22560 (98.7 % 16S rRNA gene sequence similarity), Niastella gongjuensis KACC 17399 (96.9 %), Niastella vici KCTC 42474 (96.2 %), Niastella yeongjuensis KACC 11466 (95.5 %) and Niastella koreensis KACC 11465 (95.1 %). DNA–DNA hybridization values between strain THG-YS3.2.1 and N. populi KCTC 22560, N. gongjuensis KACC 17399, N . vici KCTC 42474, N. yeongjuensis KACC 11466 and N. koreensis KACC 11465were 55.8±1.0, 39.5±0.5, 35.2±0.1, 17.6±0.3 and 12.5±1.2 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA−DNA hybridization data, strain THG-YS3.2.1 represents a novel species of the genus Niastella , for which the name Niastella hibisci sp. nov. is proposed. The type strain is THG-YS3.2.1 (=KCTC 52084=CCTCC AB 2015356).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain THG-YS3.2.1 is KT962169.

  • A supplementary table and four supplementary figures are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

  • Buck J. D..( 1982;). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. . Appl Environ Microbiol 44: 992––993.[PubMed]
    [Google Scholar]
  • Chen W. M., Lin Y. S., Sheu D. S., Sheu S. Y..( 2012;). Delftia litopenaei sp. nov., a poly-β-hydroxybutyrate-accumulating bacterium isolated from a freshwater shrimp culture pond. . Int J Syst Evol Microbiol 62: 2315––2321. [CrossRef] [PubMed]
    [Google Scholar]
  • Chen L., Wang D., Yang S. N., Wang G. J..( 2016;). Niastella vici sp. nov., isolated from farmland soil. . Int J Syst Evol Microbiol 66: 1768––1772. [CrossRef] [PubMed]
    [Google Scholar]
  • Collins M. D., Jones D..( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Bacteriol 48: 459––470. [CrossRef]
    [Google Scholar]
  • Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E..( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100: 221––230. [CrossRef] [PubMed]
    [Google Scholar]
  • Ezaki T., Hashimoto Y., Yabuuchi E..( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39: 224––229. [CrossRef]
    [Google Scholar]
  • Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17: 368––376. [CrossRef] [PubMed]
    [Google Scholar]
  • Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39: 783––791. [CrossRef]
    [Google Scholar]
  • Hall T. A..( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symposium Series 41: 95––98.
     [Google Scholar]
  • Hu H. Y., Lim B. R., Goto N., Fujie K..( 2001;). Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. . J Microbiol Methods 47: 17––24. [CrossRef] [PubMed]
    [Google Scholar]
  • Jørgensen N. O., Brandt K. K., Nybroe O., Hansen M..( 2009;). Delftia lacustris sp. nov., a peptidoglycan-degrading bacterium from fresh water, and emended description of Delftia tsuruhatensis as a peptidoglycan-degrading bacterium. . Int J Syst Evol Microbiol 59: 2195––2199. [CrossRef] [PubMed]
    [Google Scholar]
  • Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716––721. [CrossRef] [PubMed]
    [Google Scholar]
  • Kim S. J., Ahn J. H., Weon H. Y., Hong S. B., Seok S. J., Kim J. S., Kwon S. W..( 2015;). Niastella gongjuensis sp. nov., isolated from greenhouse soil. . Int J Syst Evol Microbiol 65: 3115––3118. [CrossRef] [PubMed]
    [Google Scholar]
  • Kimura M..( 1984;). The Neutral Theory of Molecular Evolution. Cambridge University Press;.
     [Google Scholar]
  • Kluge A. G., Farris J. S..( 1969;). Quantitative phyletics and the evolution of anurans. . Syst Biol 18: 1––32. [CrossRef]
    [Google Scholar]
  • Kovacs N..( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178: 703. [CrossRef] [PubMed]
    [Google Scholar]
  • Kroppenstedt R. M..( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5: 2359––2367. [CrossRef]
    [Google Scholar]
  • Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al.( 2007;). clustal w and clustal x version 2.0. . Bioinformatics 23: 2947––2948. [CrossRef] [PubMed]
    [Google Scholar]
  • Mesbah M., Premachandran U., Whitman W. B..( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39: 159––167. [CrossRef]
    [Google Scholar]
  • Minnikin D. E..( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2: 233––241. [CrossRef]
    [Google Scholar]
  • Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406––425.[PubMed]
    [Google Scholar]
  • Sasser M..( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, , MIDI Technical Note 101.. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  • Shigematsu T., Yumihara K., Ueda Y., Numaguchi M., Morimura S., Kida K..( 2003;). Delftia tsuruhatensis sp. nov., a terephthalate-assimilating bacterium isolated from activated sludge. . Int J Syst Evol Microbiol 53: 1479––1483. [CrossRef] [PubMed]
    [Google Scholar]
  • Singh H., Du J., Ngo H. T. T., Won K. H., Yang J. E., Kim K. Y., Yi T. H..( 2015;). Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant. . Antonie van Leeuwenhoek 107: 1437––1444. [CrossRef] [PubMed]
    [Google Scholar]
  • Skerman V. B. D..( 1967;). A Guide to the Identification of the Genera of Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
     [Google Scholar]
  • Stabili L., Gravili C., Tredici S. M., Piraino S., Talà A., Boero F., Alifano P..( 2008;). Epibiotic vibrio luminous bacteria isolated from some hydrozoa and bryozoa species. . Microb Ecol 56: 625––636. [CrossRef] [PubMed]
    [Google Scholar]
  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725––2729. [CrossRef] [PubMed]
    [Google Scholar]
  • Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J..( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173: 697––703.[PubMed] [CrossRef]
    [Google Scholar]
  • Weon H. Y., Kim B. Y., Yoo S. H., Lee S. Y., Kwon S. W., Go S. J., Stackebrandt E..( 2006;). Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum bacteroidetes, isolated from soil cultivated with Korean ginseng. . Int J Syst Evol Microbiol 56: 1777––1782. [CrossRef] [PubMed]
    [Google Scholar]
  • Yan Z. F., Lin P., Chu X., Kook M. C., Li C. T., Yi T. H..( 2016;). Aeromicrobium halotolerans sp. nov., isolated from desert soil sample. . Arch Microbiol 198: 423––427. [CrossRef] [PubMed]
    [Google Scholar]
  • Zhang K., Wang Y., Tang Y., Dai J., Zhang L., An H., Luo G., Rahman E., Fang C..( 2010;). Niastella populi sp. nov., isolated from soil of euphrates poplar (Populus euphratica) forest, and emended description of the genus Niastella. . Int J Syst Evol Microbiol 60: 542––545. [CrossRef] [PubMed]
    [Google Scholar]
http://sgm.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001498
Loading

Supplementary Data

 Data loading....

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001498
2016-12-01
2019-03-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5218.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001498&mimeType=html&fmt=ahah

Author and Article Information

 Figure data loading....

Footnotes:

Validated antibodies in this article

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error