1887

Abstract

A Gram-stain-negative, crescent-shaped, non-motile, aerobic bacterium was isolated from a saltern at Nari along the Bhavnagar coast, Gujarat, India. The strain designated JC344 was oxidase- and catalase-positive. The 16S rRNA gene sequence analysis and sequence comparison data indicated that JC344 represented a member of the genus and was closely related to the only species of the genus, AK24 (95.5 %). The DNA G+C content of JC344 was 43 mol%. JC344 has phosphatidylethanolamine (PE) and four unidentified polar lipids. MK-7 is the only respiratory quinone. The major (>10 %) fatty acids of strain JC344 are iso-C, anteiso-C and summed feature 9 (iso-Cω9 and/or C 10-methyl). On the basis of physiological, genotypic, phylogenetic and chemotaxonomic analyses, we conclude that JC344 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is JC344 (=KCTC 42988=LMG 29259).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001499
2016-12-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5223.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001499&mimeType=html&fmt=ahah

References

  1. Biebl H., Pfennig N. 1981; Isolation of members of the family Rhodospirillaceae . In The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria vol. 1 pp. 267–273 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer;
    [Google Scholar]
  2. Cappuccino J. G., Sherman N. 1998 Microbiology – A Laboratory Manual, 5th edn.. California: Benjamin/Cummings Science Publishing;
    [Google Scholar]
  3. Clinical and Laboratory Standards Institute 2003 Performance Standards for Antimicrobial Disks Susceptibility Tests. Approved Standard M2-A8, 8th edn. Wayne, PA: CLSI;
    [Google Scholar]
  4. Kates M. 1972 Techniques of Lipidology New York: Elsevier; [CrossRef]
    [Google Scholar]
  5. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  7. Lakshmi K. V., Sasikala Ch., Takaichi S., Ramana Ch. V. 2011; Phaeospirillum oryzae sp. nov., a spheroplast-forming, phototrophic alphaproteobacterium from a paddy soil. Int J Syst Evol Microbiol 61:1656–1661 [View Article][PubMed]
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  10. Oren A., Duker S., Ritter S. 1996; The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138:135–140 [View Article]
    [Google Scholar]
  11. Raj H. D., Maloy S. R. 1990; Proposal of C yclo bacterium marinus gen. nov., comb. nov. for a marine bacterium previously assigned to the genus Flecto bacillus . Int J Syst Bacteriol 40:337–347 [View Article]
    [Google Scholar]
  12. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  13. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Microbiology pp. 409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Srinivas T. N. R., Aditya S., Bhumika V., Kumar P. A. 2014; Lunatimonas lonarensis gen. nov., sp. nov., a haloalkaline bacterium of the family Cyclobacteriaceae with nitrate reducing activity. Syst Appl Microbiol 37:10–17 [View Article][PubMed]
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44:846–849 [View Article]
    [Google Scholar]
  17. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 3:152–155
    [Google Scholar]
  18. Tamura S., Stecher G., Tamura K. 2016; mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  19. Xie C. H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]
    [Google Scholar]
  20. Yang C. X., Liu Y. P., Bao Q. H., Feng F. Y., Liu H. R., Zhang X. J., Zhao Y. L. 2012; Mongoliitalea lutea gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a haloalkaline lake. Int J Syst Evol Microbiol 62:647–653 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001499
Loading
/content/journal/ijsem/10.1099/ijsem.0.001499
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error