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Volume 66, Issue 12

sp. nov., a psychrotolerant species widespread in natural soil and water ecosystems Free

Abstract

A novel, taxonomically unique group of six strains of the genus was discovered during an exploratory study on strains culturable from soil and water natural ecosystems in the Bohemian part of the Czech Republic. Based on the comparative analyses of the 16S rRNA gene, and sequences, these strains formed strongly supported and internally coherent clusters (intracluster identities of ≥99.9, ≥96.1 and ≥97.3 %, respectively), which were clearly separated from all known species of the genus (≤98.7, ≤83.2 and ≤88.9 %, respectively). The distinctness of the group at the species level was evidenced also by the results of the genus-wide analyses of the whole-cell mass fingerprints of the six strains generated by matrix-assisted laser desorption/ionization-time-of-flight MS and the whole-genome sequence of a group member, ANC 4603. Compared with the known species of the genus , all six strains exhibited a unique phenotype, characterized by psychrotolerance (growth at 1 °C through 28 °C), the inability to grow at 32 °C and the ability to assimilate -aspartate and malonate but not 2,3-butanediol or citrate. Based on these results, the name sp. nov. is proposed for the taxon represented by the six strains. The type strain is ANC 4603 (=CCM 8700=CCUG 69239=CNCTC 7549).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , gyrB , MALDI-TOF MS , Metabolic and physiological features , rpoB and Whole-genome sequence
© 2016 IUMS
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2016-12-01
2024-04-23
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Acinetobactercelticus sp. nov., a psychrotolerant species widespread in natural soil and water ecosystems
Int J Syst Evol Microbiol 66, 5392 (2016); https://doi.org/10.1099/ijsem.0.001526
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