%0 Journal Article %A Zhang, Long %A Chen, Xiao-Long %A Hu, Qiang %A Ruan, Zhe-Pu %A Chen, Kai %A Li, Shun-Peng %A Jiang, Jian-Dong %T Huakuichenia soli gen. nov., sp. nov., a new member of the family Microbacteriaceae, isolated from contaminated soil %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 12 %P 5399-5405 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001531 %K Gram-stain positive %K taxonomy %K 16S rRNA gene %K Huakuichenia soli gen. nov., sp. nov. %I Microbiology Society, %X A novel Gram-stain-positive, non-motile, rod-shaped bacterial strain designated LIP-1T was isolated from the contaminated soil of a pesticide factory in Xinyi, China, was investigated for its taxonomic allocation by a polyphasic approach. Cell growth occurred at 16–37 °C (optimum, 30 °C), in the presence of 0–2.0 % (w/v) NaCl (optimum, 0 %) and at pH 6.0–9.0 (optimum, pH 7.0). The major fatty acids of strain LIP-1T were anteiso-C15 : 0 (50.8 %), iso-C16 : 0 (17.6 %) and anteiso-C17 : 0 (17.4 %). The cell-wall peptidoglycan type was B2δ with 2,4-diaminobutyric acid as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and two unidentified glycolipids. The major menaquinones were MK-12 and MK-11. The genomic DNA G+C content was approximately 63.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LIP-1T formed a distinct clade within the radiation of the family Microbacteriaceae and had the highest sequence similarity with Microbacterium ginsengisoli Gsoil 259T (96.01 %) followed by Cryobacterium arcticum SK1T (94.94 %). On the basis of the phylogenetic analyses and distinct phenotypic characteristics, a new genus, namely Huakuichenia gen. nov., is proposed, harbouring the novel species Huakuichenia soli gen. nov., sp. nov. with the type strain LIP-1T (=CCTCC AB 2015422T=KCTC 39698T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001531