@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001542, author = "Song, Huiyin and Hu, Yuxin and Zhu, Huan and Wang, Qinghua and Liu, Guoxiang and Hu, Zhengyu", title = "Three novel species of coccoid green algae within the Watanabea clade (Trebouxiophyceae, Chlorophyta)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "12", pages = "5465-5477", doi = "https://doi.org/10.1099/ijsem.0.001542", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001542", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "the Watanabea clade", keywords = "Coccoid green algae", keywords = "Phylogeny", keywords = "Trebouxiophyceae", abstract = "Coccoid green algae are extremely diverse despite their simple coccoid phenotype, a phenotype that may be the result of convergent evolution. In this study, we used a polyphasic approach combining molecular phylogenetic analyses, morphology and ultrastructure to investigate isolated coccoid strains from China, and our results reveal three new lineages of Trebouxiophyceae: the novel genus and species Mysteriochloris nanningensis gen. et sp. nov., and the two novel species Phyllosiphon coccidium sp. nov. and Desertella yichangensis sp. nov. (Trebouxiophyceae, Chlorophyta). We provide a detailed characterization of the novel microalgae which they are autosporic coccoid unicells and have parietal chloroplasts. In phylogenies based on 18S rDNA sequences and the chloroplast ribulose-bisphosphate carboxylase gene (rbcL), these three algae are nested within the Watanabea clade and are different from any known algae. M. nanningensis FACHB-1787 is not really close to any known algae within the Watanabea clade. Phyllosiphoncoccidium FACHB-2212 is within the Phyllosiphon lineages. D. yichangensis FACHB-1793 is closely related to Desertella californica and described as a representative of a novel species of the genus Desertella.", }