1887

Abstract

A novel actinomycete, designated strain KC-035, was isolated from soil collected from Krung Ching Waterfall National Park, Nakhon Si Thammarat Province, Thailand. Its taxonomic position was determined using a polyphasic approach. The strain had morphological and chemotaxonomic properties typical of members of the genus : flexuous spore chain; -diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H), MK-9(H) and MK-9(H) as menaquinones; diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as phospholipids; anteiso-C, C, iso-C, iso-C and iso-C as major cellular fatty acids; and DNA G+C content of 72 mol%. 16S rRNA gene sequence analysis revealed that strain KC-035 showed high similarity to n20 (99.16 %) and KC-038 (98.43 %) as well as formed a monophyletic clade with them in the phylogenetic tree. On the basis of comparison of phenotypic properties and the low level of DNA–DNA relatedness, strain KC-035 could be distinguished from its closely related species and is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KC-035 (=NBRC 110087=KCTC 29503=TISTR 2402).

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2017-01-01
2024-04-10
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