%0 Journal Article %A Park, Miri %A Joung, Yochan %A Nam, Gi Gyun %A Kim, Suhyun %A Cho, Jang-Cheon %T Flavobacterium inkyongense sp. nov., isolated from an artificial freshwater pond %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 1 %P 82-86 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001575 %K Flavobacterium %K taxonomy %K Flavobacterium inkyongense %K freshwater %I Microbiology Society, %X A Gram-stain-negative, non-motile, yellow-pigmented bacterium, designated strain IMCC27201T, was isolated from an artificial freshwater pond (Inkyong) in Korea. Growth of strain IMCC27201T occurred at 10–37 °C (optimum, 30 °C), at pH 7.0–8.0 (optimum, pH 7.0) and with 0–0.25 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain IMCC27201T belonged to the genus Flavobacterium and was related most closely to Flavobacterium columnare IFO 15943T (97.8 % sequence similarity), Flavobacterium terrae R2A1-13T (97.2 %) and Flavobacterium vireti THG-SM1T (96.4 %). Strain IMCC27201T exhibited low DNA–DNA relatedness with F. columnare KCTC 42885T (34.9–40.6 %). The DNA G+C content of strain IMCC27201T was 32.1 mol%. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C15 : 0 3-OH. Strain IMCC27201T contained menaquinone-6 (MK-6) as the sole isoprenoid quinone. The polar lipids of strain IMCC27201T consisted of phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Based on 16S rRNA gene phylogeny, phenotypic characterization and DNA–DNA relatedness, strain IMCC27201Trepresents a novel species of the genus Flavobacterium , for which the name Flavobacterium inkyongense sp. nov. is proposed. The type strain is IMCC27201T (=KCTC 52244T=JCM 31385T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001575