%0 Journal Article %A Song, Jinlong %A Wang, Yanwei %A Song, Yi %A Zhao, Bingqiang %A Wang, Huimin %A Zhou, Shan %A Kong, Delong %A Guo, Xiang %A Li, Yanting %A He, Mingxiong %A Ma, Kedong %A Ruan, Zhiyong %A Yan, Yanchun %T Brevibacillus halotolerans sp. nov., isolated from saline soil of a paddy field %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 4 %P 772-777 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001579 %K saline soil %K 16S rRNA gene %K polyphasic taxonomy %K paddy field %K Brevibacillus halotolerans sp. nov. %I Microbiology Society, %X Two novel aerobic bacteria, designated strains LAM0312T and LAM0313, were isolated from saline soil samples collected from a paddy field in Dezhou city, Shandong Province, China. Cells of these strains were Gram-stain-positive, sporogenous, rod-shaped and motile with peritrichous flagella. The optimal growth temperature and pH were 30 °C and pH 7.0–8.0. Strain LAM0312T was able to grow in the presence of 12 % (w/v) NaCl. The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The dominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipids, five unidentitied lipids and phosphatidylmonomethylethanolamine. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as menaquinone-7. The G+C content of the genomic DNA of strains LAM0312T and LAM0313 was 45.0 and 46.0 mol%, respectively, as determined by the T m method. 16S rRNA gene sequence similarity analysis indicated that the strains were closely related to Brevibacillus laterosporus DSM 25T and Brevibacillus fluminis JCM 15716T with 98.5 and 96.4 % sequence similarity, respectively. The DNA–DNA hybridization value between strain LAM0312T and LAM0313 was 92±0.6 % (reciprocal 90±0.2 %) and the value between strain LAM0312T and Brevibacillus laterosporus DSM 25T was 48±0.5 % (reciprocal 40±0.4 %). On the basis of the phenotypic, phylogenetic and chemotaxonomic characteristics, the two strains are proposed to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus halotolerans sp. nov. is proposed. The type strain is LAM0312T (=ACCC 06527T=JCM 30849T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001579