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f Soortia roseihalophila gen. nov., sp. nov., a new taxon in the order Balneolales isolated from a travertine spring, and description of Soortiaceae fam. nov.

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Soortia roseihalophila gen. nov., sp. nov., a new taxon in the order Balneolales isolated from a travertine spring, and description of Soortiaceae fam. nov., Page 1 of 1

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  • A novel Gram-stain-negative, slightly halophilic, motile, curved rod with a horseshoe shape, designated strain Bsw-2b, was isolated from Badab-Soort travertine spring in Iran. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Bsw-2b belongs to the order Balneolales , showing 84.6 % sequence similarity to Gracilimonas tropica DSM 19535 and 84.4 % and 83.9 % sequence similarity to Gracilimonas rosea CL-KR2 and Balneola vulgaris DSM 17893, respectively. In addition, phenotypic and physiological features could clearly differentiate strain Bsw-2b from species of the most closely related genera, Gracilimonas , Balneola , Aliifodinibius and Fodinibius . The strain was able to grow with 1–3 % (w/v) (optimum at 2 %) NaCl, at temperatures of 28–34 °C (optimum at 30 °C) and between pH 6.0 and 8.0 (optimum at pH 7.0). The major cellular fatty acids of strain Bsw-2b were iso-C15 : 0, iso-C13 : 0 and iso-C14 : 0. The polar lipid profile of strain Bsw-2b was composed predominantly of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and four unknown phospholipids. The DNA G+C content was 40.5 mol%. Based on the evidence from the polyphasic study, strain Bsw-2b represents a novel species in a novel genus within a new family, for which the name Soortia roseihalophila gen. nov., sp. nov. is proposed, within the new family Soortiaceae fam. nov. The type strain is strain Bsw-2b (=IBRC-M 10915=LMG 28547).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Bsw-2b is KU695464.

  • One supplementary figure is available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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2017-02-20
2019-02-24
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