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f Streptomyces capparidis sp. nov., a novel endophytic actinobacterium isolated from fruits of Capparis spinosa L.

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  • Authors: Hong-Fei Wang1​,2​ ,  Qiu-Li Li1​ ,  Min Xiao3​ ,  Yong-Guang Zhang2​ ,  Xing-Kui Zhou4​ ,  Manik Prabhu Narsing Rao3​ ,  Yan-Qing Duan4​ , Wen-Jun Li2​,3​
    • VIEW AFFILIATIONS
    • 1​ 1​College of Life Science, Liaoning Normal University, Dalian 116029, PR China 2​ 2​Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China 3​ 3​State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China 4​ 4​China Tobacco Yunnan Industrial Co., Ltd, Kunming 650231, PR China
  • *Correspondence: Wen-Jun Li, [email protected]
  • First Published Online: 20 February 2017, International Journal of Systematic and Evolutionary Microbiology 67: 133-137, doi: 10.1099/ijsem.0.001586
  • Subject: New taxa - Actinobacteria
  • Received:
  • Accepted:
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Streptomyces capparidis sp. nov., a novel endophytic actinobacterium isolated from fruits of Capparis spinosa L., Page 1 of 1

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  • A novel endophytic actinobacterial strain, designated EGI 6500195, was isolated from fruits of Capparis spinosa. Growth occurred at 10–45 °C (optimum 30 °C), at pH 6–8 (optimum pH 7) and in the presence of 0–1 % (w/v) NaCl. Strain EGI 6500195 shared highest 16S rRNA gene sequence similarity (97.74 %) with Streptomyces vitaminophilus DSM 41686 and less than 97 % sequence similarity with other members of the genus Streptomyces . The diagnostic amino acid in the peptidoglycan was ll-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose, fructose and mannose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The polar lipid profile of strain EGI 6500195 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylcholine, three unknown phospholipids, an unknown aminophospholipid and an unknown aminolipid. The cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 1 ω9c, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and iso-C17 : 1 ω9c. The DNA G+C content of strain EGI 6500195 was 74.1 mol%. The level of DNA–DNA relatedness between strain EGI 6500195 and Streptomyces. vitaminophilus DSM 41686 was 14.1±3.5 %. On the basis of the phenotypic, phylogenetic, chemotaxonomic and DNA–DNA hybridization data, strain EGI 6500195 represents a novel species of the genus Streptomyces , for which the name Streptomyces capparidis sp. nov. is proposed. The type strain is EGI 6500195 (=DSM 42145=JCM 30089).

  • The 16S rRNA gene sequence of strain EGI 6500195 has been deposited in GenBank under the accession number KX119420.

  • Three supplementary figures and three supplementary tables are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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