1887

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Volume 67, Issue 2

sp. nov., a moderately halophilic bacterium isolated from a tidal flat environment Free

Abstract

Two Gram-stain-negative, aerobic, motile, halophilic, rod-shaped bacteria, designated Hb8 and Hb20, were isolated from a tidal flat environment located on the South-West Korean peninsula. The isolates grew at 10–37 °C, at pH 5.0–9.0 and in NaCl concentrations of 0.5–15 % (w/v; optimum, 3.0–6.0 %). Sequence analysis of the 16S rRNA indicated that the isolates belong to the genus and are most closely related to R65 (98.3 %), followed by SM19, SD-14B and A3d10. The overall 16S rRNA gene sequence similarity with these species was 97.9 %, but Hb8 and Hb20 showed 100 % sequence similarity with each other. DNA–DNA relatedness values of H8 and Hb20 suggested that these isolates represent a single species, while DNA–DNA relatedness values of the two novel isolates with DSM 27079 and DSM 15400 were only 21.3 and 22.9 %, respectively. The major fatty acids present in strain Hb8 were identified as C, Cω, Cω, C 3-OH and summed feature 3 (Cω and/or Cω). Ubiquinone-9 was the main respiratory quinone in both the novel strains. The polar lipids found to be present included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids and five unidentified lipids. The genomic DNA G+C content of Hb8 and Hb20 was 54.5 mol%. Polyphasic analysis indicated that the two isolates are representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Hb8 (=KCTC 52255=JCM 31416).

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Keyword(s): halophilic , marine sediment , Marinobacter salinus , novel species and polyphasic
© 2017 IUMS
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2017-02-01
2024-04-19
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Marinobacter salinus sp. nov., a moderately halophilic bacterium isolated from a tidal flat environment
Int J Syst Evol Microbiol 67, 205 (2017); https://doi.org/10.1099/ijsem.0.001587
/content/journal/ijsem/10.1099/ijsem.0.001587
/content/journal/ijsem/10.1099/ijsem.0.001587
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