1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 3

sp. nov., isolated from the rhizosphere of L. Free

Abstract

A Gram-stain-negative, smooth, bright-yellow-pigmented, rod-shaped bacterial strain, slightly motile by gliding, catalase- and oxidase-positive and aerobic, but growing weakly under anaerobic conditions, was isolated from the rhizosphere of the flower mugunghwa ( L.) located in Kyung Hee University, Yongin, Gyeonggi, South Korea. The strain named THG-HG1.4 grew at 15–35 °C, pH 6.5–9.0 and in the presence of 0–2.5 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequence showed that strain THG-HG1.4 was most closely related to HME7524 (98.83 %) and S2-3H (97.28 %). The DNA G+C content of strain THG-HG1.4 was 41.2 mol%. In DNA–DNA hybridization, the DNA–DNA relatedness between strain THG-HG1.4 and its closest phylogenetic neighbour was below 64.1 %. The predominant isoprenoid quinone detected in strain THG-HG1.4 was menaquinone-6 (MK-6). The major polar lipids were found to be phosphatidylethanolamine, three unidentified lipids, two unidentified glycolipids and an unidentified aminolipid. The major fatty acids were identified as iso-C, iso-C 3-OH, C, iso-C 3-OH and summed feature 3. Thus, based on the report of the phenotypic, genotypic and phylogenetic characterization of strain THG-HG1.4, it has been concluded that the novel isolate represents a novel species of the genus sp. nov. is proposed, with THG-HG1.4 (=KACC 18852=CCTCC AB 2016178) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Flavobacterium hibisci , Hibiscus syriacus L. , Mugunghwa flower and phenotipic analysis
© 2017 IUMS
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2017-03-01
2024-04-20
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Flavobacterium hibisci sp. nov., isolated from the rhizosphere of Hibiscus syriacus L.
Int J Syst Evol Microbiol 67, 537 (2017); https://doi.org/10.1099/ijsem.0.001610
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