1887

Abstract

A Gram-staining-negative, strictly aerobic bacterial strain, designated MA7-20, was isolated from a marine alga, , in Korea. Cells showing oxidase-positive and catalase-positive activities were motile rods with bipolar flagella. Growth of strain MA7-20 was observed at 15–45 °C (optimum, 30–37 °C), at pH 6.0–10.5 (optimum, pH 7.0–8.0) and in the presence of 0–7 % (w/v) NaCl (optimum, 2–3 %). Strain MA7-20 contained summed feature 8 (comprising Cω7/C ω6), 11-methyl Cω7 and C as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidyl--methylethanolamine. The G+C content of the genomic DNA was 61.5 mol%. Strain MA7-20 was most closely related to YC6898, CC-AMH-0 and J3 with 96.0, 95.8 and 95.8 % 16S rRNA gene sequence similarities, respectively, but the strain formed a distinct phylogenetic lineage from them within the family with a low bootstrap value. also formed a clearly distinct phylogenetic lineage from other members of the genus and closely related genera. On the basis of phenotypic, chemotaxonomic and molecular properties, strain MA7-20 represents a novel species of a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is MA7-20 (=KACC 18807=JCM 31538). In addition, is also reclassified as gen. nov., comb. nov. (type strain YC6898=KACC 14911=NBRC 107700).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001633
2017-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/2/362.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001633&mimeType=html&fmt=ahah

References

  1. Mergaert J, Swings J. Family IV. Phyllobacteriaceae fam. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. Bergey’s Manual of Systematic Bacteriology (The Proteobacteria), part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria) , 2nd ed. vol. 2 New York: Springer; 2005 p 393
    [Google Scholar]
  2. Mergaert J, Swings J. Family IV. Phyllobacteriaceae fam. nov. In list of new names and new combinations previously effectively, but not validly published, validation list no. 107. Int J Syst Evol Microbiol 2006; 56:1–6 [CrossRef]
    [Google Scholar]
  3. Roh SW, Kim KH, Nam YD, Chang HW, Kim MS et al. Aliihoeflea aestuarii gen. nov., sp. nov., a novel bacterium isolated from tidal flat sediment. J Microbiol 2008; 46:594–598 [View Article][PubMed]
    [Google Scholar]
  4. Kampfer P, Neef A, Salkinoja-Salonen MS, Buss HJ. Chelatobacter heintzii (Auling et al. 1993) is a later subjective synonym of Aminobacter aminovorans (Urakami et al. 1992). Int J Syst Evol Microbiol 2002; 52:835–839 [View Article][PubMed]
    [Google Scholar]
  5. Bambauer A, Rainey FA, Stackebrandt E, Winter J. Characterization of Aquamicrobium defluvii gen. nov. sp. nov., a thiophene-2-carboxylate-metabolizing bacterium from activated sludge. Arch Microbiol 1998; 169:293–302[PubMed] [CrossRef]
    [Google Scholar]
  6. Doronina NV, Kaparullina EN, Trotsenko YA, Nörtemann B, Bucheli-Witschel M et al. Chelativorans multitrophicus gen. nov., sp. nov. and Chelativorans oligotrophicus sp. nov., aerobic EDTA-degrading bacteria. Int J Syst Evol Microbiol 2010; 60:1044–1051 [View Article][PubMed]
    [Google Scholar]
  7. Li Y, Piao CG, Guo LM, Chang JP, Li X et al. Corticibacterium populi gen. nov., sp. nov., a member of the family Phyllobacteriaceae, isolated from bark of Populus×euramericana. Int J Syst Evol Microbiol 2016; 66:2617–2622 [View Article]
    [Google Scholar]
  8. Peix A, Rivas R, Trujillo ME, Vancanneyt M, Velázquez E et al. Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov. Int J Syst Evol Microbiol 2005; 55:1163–1166 [View Article][PubMed]
    [Google Scholar]
  9. Park S, Lee JS, Lee KC, Yoon JH. Lentilitoribacter donghaensis gen. nov., sp. nov., a slowly-growing alphaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek 2013; 103:457–464 [View Article][PubMed]
    [Google Scholar]
  10. Jarvis BDW, van Berkum P, Chen WX, Nour SM, Fernandez MP et al. Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 1997; 47:895–898 [CrossRef]
    [Google Scholar]
  11. Labbé N, Parent S, Villemur R. Nitratireductor aquibiodomus gen. nov., sp. nov., a novel α-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol 2004; 54:269–273 [View Article][PubMed]
    [Google Scholar]
  12. Hameed A, Shahina M, Lai WA, Lin SY, Young LS et al. Oricola cellulosilytica gen. nov., sp. nov., a cellulose-degrading bacterium of the family Phyllobacteriaceae isolated from surface seashore water, and emended descriptions of Mesorhizobium loti and Phyllobacterium myrsinacearum. Antonie van Leeuwenhoek 2015; 107:759–771 [View Article][PubMed]
    [Google Scholar]
  13. Luo XS, Pan YF, Shi JJ, Dai J, Hou S et al. Evidence of the presence of bacteria highly resistant to beta-lactam antibiotics in Taklimakan Desert and biochemical characterization of Paramesorhizobium deserti gen. nov., sp. nov. Prog Biochem Biophys 2015; 42:56–64
    [Google Scholar]
  14. Knösel DH. Prufung von Bakterien auf Fahigkeit zur Sternbildung. Zentralbl Bakteriol Parasirtenkd Infektionskr Hyg Abt 1962; 2:79–100
    [Google Scholar]
  15. Jung YT, Park S, Lee JS, Oh TK, Yoon JH. Pseudahrensia aquimaris gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012; 62:2056–2061 [View Article][PubMed]
    [Google Scholar]
  16. Kämpfer P, Müller C, Mau M, Neef A, Auling G et al. Description of Pseudaminobacter gen. nov. with two new species, Pseudaminobacter salicylatoxidans sp. nov. and Pseudaminobacter defluvii sp. nov. Int J Syst Bacteriol 1999; 49:887–897 [View Article][PubMed]
    [Google Scholar]
  17. Han L, Mo Y, Feng Q, Zhang R, Zhao X et al. Tianweitania sediminis gen. nov., sp. nov., a member of the family Phyllobacteriaceae, isolated from subsurface sediment core. Int J Syst Evol Microbiol 2016; 66:719–724 [View Article][PubMed]
    [Google Scholar]
  18. Murphy CD, Moore RM, White RL. Peroxidases from marine microalgae. J Appl Phycol 2000; 12:507–513 [CrossRef]
    [Google Scholar]
  19. Kim JM, Le NT, Chung BS, Park JH, Bae JW et al. Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 2008; 74:7313–7320 [View Article][PubMed]
    [Google Scholar]
  20. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  21. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007; 3:e56 [View Article][PubMed]
    [Google Scholar]
  22. Felsenstein J. Phylip (Phylogeny Inference Package), Version 3.6a Seattle, WA: Department of Genetics, University of Washington; 2002
    [Google Scholar]
  23. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 9:1312–1313 [CrossRef]
    [Google Scholar]
  24. Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 2007; 73:5261–5267 [View Article][PubMed]
    [Google Scholar]
  25. Gomori G. Preparation of buffers for use in enzyme studies. In Colowick SP, Kaplan NO. (editors) Methods in Enzymology New York: Academic Press; 1955 pp 138–146
    [Google Scholar]
  26. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. editor Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
    [Google Scholar]
  27. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  28. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  29. Lányi B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67 [CrossRef]
    [Google Scholar]
  30. Jeong SH, Park MS, Jin HM, Lee K, Park W et al. Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana. Int J Syst Evol Microbiol 2013; 63:332–338 [View Article][PubMed]
    [Google Scholar]
  31. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [CrossRef]
    [Google Scholar]
  32. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–208 [CrossRef]
    [Google Scholar]
  33. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773[PubMed] [CrossRef]
    [Google Scholar]
  34. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  35. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [CrossRef]
    [Google Scholar]
  36. Jang GI, Hwang CY, Cho BC. Nitratireductor aquimarinus sp. nov., isolated from a culture of the diatom Skeletonema costatum, and emended description of the genus Nitratireductor. Int J Syst Evol Microbiol 2011; 61:2676–2681 [View Article][PubMed]
    [Google Scholar]
  37. Chung EJ, Park JA, Pramanik P, Bibi F, Jeon CO et al. Hoeflea suaedae sp. nov., an endophytic bacterium isolated from the root of the halophyte Suaeda maritima. Int J Syst Evol Microbiol 2013; 63:2277–2281 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001633
Loading
/content/journal/ijsem/10.1099/ijsem.0.001633
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error