1887

Abstract

The morphology and infraciliature of one freshwater ciliate, spec. nov., isolated from a farmland pond in Harbin, northeastern China, was investigated using living observation and silver staining methods. spec. nov. could be distinguished by the following features: body approximately 20–25×10–15 µm ; buccal field about 45–50 % of body length; 11 somatic kineties; somatic kinety n terminating sub-caudally; two macronuclei and one micronucleus; M1 almost as long as M2; M2 triangle-shaped. The genus is re-defined as follows: body outline usually oval or elliptical, ventral side concave, dorsal side convex; single caudal cilium; contractile vacuole posterior terminal; adoral membranelles usually not separated; paroral membrane ‘L’-shaped, with anterior end terminating at the level of anterior end of M1; somatic kineties longitudinally arranged and continuous. Phylogenetic trees based on the SSU rDNA sequences showed that spec. nov. clusters with the type species, , with full support. is not monophyletic with members of the clade of +++.

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2017-03-01
2024-03-29
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References

  1. Budiño B, Lamas J, Pata MP, Arranz JA, Sanmartín ML et al. Intraspecific variability in several isolates of Philasterides dicentrarchi (syn. Miamiensis avidus), a scuticociliate parasite of farmed turbot. Vet Parasitol 2011; 175:260–272 [View Article][PubMed]
    [Google Scholar]
  2. De Castro LA, Küppers GC, Schweikert M, Harada ML, Paiva TS. Ciliates from eutrophized water in the northern Brazil and morphology of Cristigera hammeri Wilbert, 1986 (Ciliophora, Scuticociliatia). Eur J Protistol 2014; 50:122–133 [View Article][PubMed]
    [Google Scholar]
  3. Fan X, Hu X, Al-Farraj SA, Clamp JC, Song W. Morphological description of three marine ciliates (Ciliophora, Scuticociliatia), with establishment of a new genus and two new species. Eur J Protistol 2011; 47:186–196 [View Article][PubMed]
    [Google Scholar]
  4. Fan X, Lin X, Al-Rasheid KAS, Warren A, Song W. The diversity of scuticociliates (Protozoa, Ciliophora): a report on eight marine forms found in coastal waters of China, with a description of one new species. Acta Protozool 2011; 50:219–234
    [Google Scholar]
  5. Foissner W, Jung JH, Filker S, Rudolph J, Stoeck T. Morphology, ontogenesis and molecular phylogeny of Platynematum salinarum nov. spec., a new scuticociliate (Ciliophora, Scuticociliatia) from a solar saltern. Eur J Protistol 2014; 50:174–184 [View Article][PubMed]
    [Google Scholar]
  6. Foissner W, Wilbert N. A comparative study of the infraciliature and silverline system of the fresh-water scuticociliates Pseudocohnilembus putrinus (Kahl, 1928) nov. comb., P. pusillus (Quennerstedt, 1869) nov. comb., and the marine form P. marinus Thompson, 19661. J Protozool 1981; 28:291–297 [View Article]
    [Google Scholar]
  7. Lynn DH, Strüder-Kypke M. Scuticociliate endosymbionts of echinoids (phylum Echinodermata): phylogenetic relationships among species in the genera Entodiscus, Plagiopyliella, Thyrophylax, and Entorhipidium (phylum Ciliophora). J Parasitol 2005; 91:1190–1199 [View Article][PubMed]
    [Google Scholar]
  8. Pan H, Hu J, Warren A, Wang L, Jiang J et al. Morphology and molecular phylogeny of Pleuronema orientale spec. nov. and Pleuronema paucisaetosum spec. nov. (Ciliophora, Scuticociliata) from Hangzhou Bay, China. Int J Syst Evol Microbiol 2015; 65:4800–4808 [View Article][PubMed]
    [Google Scholar]
  9. Pan X, Bourland WA, Song W. Protargol synthesis: an in-house protocol. J Eukaryot Microbiol 2013; 60:609–614 [View Article][PubMed]
    [Google Scholar]
  10. Pan X, Zhu M, Ma H, Al-Rasheid KA, Hu X. Morphology and small-subunit rRNA gene sequences of two novel marine ciliates, Metanophrys orientalis spec. nov. and Uronemella sinensis spec. nov. (Protista, Ciliophora, Scuticociliatia), with an improved diagnosis of the genus Uronemella. Int J Syst Evol Microbiol 2013; 63:3515–3523 [View Article][PubMed]
    [Google Scholar]
  11. Pan X, Huang J, Gao F, Fan X, Ma H et al. Morphology and phylogeny of four marine scuticociliates (Protista, Ciliophora), with descriptions of two new species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov. Acta Protozool 2015; 54:31–43
    [Google Scholar]
  12. Pan X, Yi Z, Li J, Ma H, Al-Farraj SA et al. Biodiversity of marine scuticociliates (Protozoa, Ciliophora) from China: description of seven morphotypes including a new species, Philaster sinensis spec. nov. Eur J Protistol 2015; 51:142–157 [View Article][PubMed]
    [Google Scholar]
  13. Pan X, Fan X, Al-Farraj SA, Gao S, Chen Y. Taxonomy and morphology of four “ophrys-related” scuticociliates (Protista, Ciliophora, Scuticociliatia), with description of a new genus, Paramesanophrys gen. nov. Eur J Taxon 2016; 191:1–18
    [Google Scholar]
  14. Pan X, Bullard SA. Seven scuticociliates (Protozoa, Ciliophora) from Alabama, USA, with descriptions of two parasitic species isolated from a freshwater mussel Potamilus purpuratus. Eur J Taxon 2016 in press [View Article]
    [Google Scholar]
  15. Song W, Shang H, Chen Z, Ma H. Comparison of some closely-related Metanophrys-taxa with description of a new species Metanophrys similis nov. spec. (Ciliophora, Scuticociliatida). Eur J Protistol 2002; 38:45–53 [View Article]
    [Google Scholar]
  16. Song W, Zhao Y, Xu K, Hu X, Gong J et al. Pathogenic Protozoa in Mariculture Beijing: Science Press; 2003
    [Google Scholar]
  17. Song W, Warren A, Hu X. (editors) Free-Living Ciliates in Bohai and Yellow Sea, China Beijing: Science Press; 2009
    [Google Scholar]
  18. Zhan Z, Stoeck T, Dunthorn M, Xu K. Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization. Eur J Protistol 2014; 50:16–24 [View Article][PubMed]
    [Google Scholar]
  19. Mallo N, Lamas J, Piazzon C, Leiro JM. Presence of a plant-like proton-translocating pyrophosphatase in a scuticociliate parasite and its role as a possible drug target. Parasitology 2015; 142:449–462 [View Article][PubMed]
    [Google Scholar]
  20. Ofelio C, Blanco A, Roura Á, Pintado J, Pascual S et al. Isolation and molecular identification of the scuticociliate Porpostoma notata Moebius, 1888 from moribund reared Hippocampus hippocampus (L.) seahorses, by amplification of the SSU rRNA gene sequences. J Fish Dis 2014; 37:1061–1065 [View Article][PubMed]
    [Google Scholar]
  21. Perez-Uz B, Song W. Uronema gallicum sp. n. (Protozoa: Ciliophora) a new marine scuticociliate from the coastal area of Calais. Acta Protozool 1995; 34:143–149
    [Google Scholar]
  22. Song W. Morphological and taxonomical studies on some marine scuticociliates from China sea, with description of two new species, Philasterides armatalis sp. n. and Cyclidium varibonneti sp. n. (Protozoa: Ciliophora: Scuticociliatida). Acta Protozool 2000; 39:295–322
    [Google Scholar]
  23. Song W, Wilbert N. Redefinition and redescription of some marine scuticociliates from China, with report of a new species, Metanophrys sinensis nov. spec. (Ciliophora, Scuticociliatida). Zool Anz 2000; 239:45–74
    [Google Scholar]
  24. Song W, Wilbert N. Reinvestigations of three “well-known” marine scuticociliates: Uronemella filificum (Kahl, 1931) nov. gen., nov. comb., Pseud cohnilembus hargisi Evans & Thompson, 1964 and Cyclidium citrullus Cohn 1865, with Description of the New Genus Uronemella (Protozoa, Ciliophora, Scuticociliatida). Zool Anz - J Comp Zool 2002; 241:317–331 [View Article]
    [Google Scholar]
  25. Fan X, Miao M, Al-Rasheid KA, Song W. A new genus of marine scuticociliate (Protozoa, Ciliophora) from northern China, with a brief note on its phylogenetic position inferred from small subunit ribosomal DNA sequence data. J Eukaryot Microbiol 2009; 56:577–582 [View Article][PubMed]
    [Google Scholar]
  26. Fan X, Chen X, Song W, Al-Rasheid KA, Warren A. Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., with improved definition of Pseudoplatynematum (Ciliophora, Oligohymenophora). Eur J Protistol 2010; 46:212–220 [View Article][PubMed]
    [Google Scholar]
  27. Gao F, Fan X, Yi Z, Strüder-Kypke M, Song W. Phylogenetic consideration of two Scuticociliate Genera, Philasterides and Boveria (Protozoa, Ciliophora) based on 18S rRNA gene sequences. Parasitol Int 2010; 59:549–555 [View Article][PubMed]
    [Google Scholar]
  28. Gao F, Katz LA, Song W. Insights into the phylogenetic and taxonomy of philasterid ciliates (Protozoa, Ciliophora, Scuticociliatia) based on analyses of multiple molecular markers. Mol Phylogenet Evol 2012; 64:308–317 [View Article][PubMed]
    [Google Scholar]
  29. Gao F, Strüder-Kypke M, Yi Z, Miao M, Al-Farraj SA et al. Phylogenetic analysis and taxonomic distinction of six genera of pathogenic scuticociliates (Protozoa, Ciliophora) inferred from small-subunit rRNA gene sequences. Int J Syst Evol Microbiol 2012; 62:246–256 [View Article][PubMed]
    [Google Scholar]
  30. Gao F, Katz LA, Song W. Multigene-based analyses on evolutionary phylogeny of two controversial ciliate orders: Pleuronematida and Loxocephalida (Protista, Ciliophora, Oligohymenophorea). Mol Phylogenet Evol 2013; 68:55–63 [View Article][PubMed]
    [Google Scholar]
  31. Gao F, Gao S, Wang P, Katz LA, Song W. Phylogenetic analyses of cyclidiids (Protista, Ciliophora, Scuticociliatia) based on multiple genes suggest their close relationship with thigmotrichids. Mol Phylogenet Evol 2014; 75:219–226 [View Article][PubMed]
    [Google Scholar]
  32. Pan H, Huang J, Hu X, Fan X, Al-Rasheid KAS et al. Morphology and SSU rRNA gene sequences of three marine ciliates from Yellow Sea, China, including one new species, Uronema heteromarinum nov. spec. (Ciliophora, Scuticociliatida). Acta Protozool 2010; 49:45–49
    [Google Scholar]
  33. Pan X, Shao C, Ma H, Fan X, Al-Rasheid KAS et al. Redescriptions of two marine scuticociliates from China, with notes on stomatogenesis in Parauronema longum (Ciliophora, Scuticociliatida). Acta Protozool 2011; 50:301–310
    [Google Scholar]
  34. Agamaliev FG. Ciliates of the Caspian Sea. Taxonomy, ecology, zoogeography. Nauka, Leningrad (in Russian); 1983
  35. Alekperov IK. Atlas svobodnozhivushchikh infuzorii (Atlas of Free-Living Ciliates), Baku: Vorcali NRM; 2005
  36. Borror AC. Tidal marsh ciliates (Protozoa): morphology, ecology, systematics. Acta Protozool 1972; 10:29–71
    [Google Scholar]
  37. Didier P, Wilbert N. Sur un Cyclidium glaucoma de la région de bonn (R.F.A.). Archiv für Protistenkunde 1981; 124:96–102 [View Article]
    [Google Scholar]
  38. Grolière CA. Morphologie et stomatogenèse chez deux ciliés Scuticociliatida des genres Philasterides Kahl, 1926 et Cyclidium O. F. Müller, 1786. Acta Protozool 1980; 19:195–206
    [Google Scholar]
  39. Song W, Wei J. Morphological studies on three marine pathogenic ciliates (Protozoa, Ciliophora). Acta Hydrobiol Sin 1998; 22:361–366
    [Google Scholar]
  40. Müller OF. Dnimalcula Infusoria Fluviatilia et Marina, etc. Havniae; 1786
  41. Foissner W, Berger H, Kohmann F. Taxonomische und ökologische revision der ciliaten des saprobiensystems. Band III: Hymenostomata, Prostomatida, Nassulida. Informationsberichte des Bayerischen Landesamtes für Wasserwirtschaft, Heft 1/94; 19941–548
  42. Wilbert N. Eine Verbesserte technik der protargolimprägnation für ciliaten. Mikrokosmos 1975; 64:171–179
    [Google Scholar]
  43. Ma H, Choi JK, Song W. An improved silver carbonate impregnation for marine ciliated protozoa. Acta Protozool 2003; 95:431–519
    [Google Scholar]
  44. Wilbert N, Song W. A further study on littoral ciliates (Protozoa, Ciliophora) near King George Island, Antarctica, with description of a new genus and seven new species. J Nat Hist 2008; 42:979–1012 [View Article]
    [Google Scholar]
  45. Lynn DH. The Ciliated Protozoa: Characterization, Classification and Guide to the Literature, 3rd ed. Dordrecht: Springer; 2008 pp. 1–605
    [Google Scholar]
  46. Medlin L, Elwood HJ, Stickel S, Sogin ML. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 1988; 71:491–499 [View Article][PubMed]
    [Google Scholar]
  47. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  48. Stamatakis A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 2006; 22:2688–2690 [View Article][PubMed]
    [Google Scholar]
  49. Stamatakis A, Hoover P, Rougemont J. A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 2008; 57:758–771 [View Article][PubMed]
    [Google Scholar]
  50. Posada D, Crandall KA. MODELTEST: testing the model of DNA substitution. Bioinformatics 1998; 14:817–818 [View Article][PubMed]
    [Google Scholar]
  51. Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003; 19:1572–1574 [View Article][PubMed]
    [Google Scholar]
  52. Nylander JAA. MrModeltest v2. distributed by the author. Department of Systematic Zoology, Evolutionary Biology Centre, Uppsala University; 2004
  53. Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 1996; 12:357–358[PubMed]
    [Google Scholar]
  54. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  55. Berger J, Thompson JC. A redescription of Cyclidium glaucoma O.F.M., 1786 (Ciliata: Hymenostomatida), with particular attention to the buccal apparatus. J Protozool 1960; 7:256–262 [View Article]
    [Google Scholar]
  56. Dragesco J. Compléments à la connaissance des ciliés mésopsammiques de Roscoff. 1. Holotriches. Cah Biol Mar 1963; 4:91–119
    [Google Scholar]
  57. Wilbert N. Ciliaten aus dem interstitial des Ontario Sees. Acta Protozool 1986; 25:379–396
    [Google Scholar]
  58. Small EB, Lynn DH. Phylum ciliophora doflein, 1901. In Lee JJ, Hutner SH, Bovee EC. (editors) An Illustrated Guide to the Protozoa Kansas: Society of Protozoologists, Allen Press; 1985 pp. 393–575
    [Google Scholar]
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