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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 3

gen. nov., sp. nov., a diazotrophic and facultatively methylotrophic bacterium, isolated from rice rhizosphere Free

Abstract

A novel facultatively methanol-utilizing bacterial strain, SM30, was isolated from rice rhizosphere. Strain SM30 was Gram-stain-negative, aerobic, motile, short rods, and grew optimally at pH 7 and at 28 °C. It could tolerate 0 to 2 % (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain SM30 was most closely related to DSM 16300, with a low similarity of 94.17 %. One of the lanthanide metals, lanthanum, could enhance its growth slightly on methanol. Phylogenetic trees, based on the , and genes of SM30 showed its distinct phylogenetic position with respect to species with validly published names. Polymerase chain reaction (PCR) amplification of the and growth on nitrogen-free medium indicated that strain SM30 is a diazotroph. The major cellular fatty acids were summed feature 8 (containing 18 : 1ω7 and 18 : 1ω6) and cyclo 19 : 0ω8. The major quinone was ubiquinone 10. The DNA G+C content was 74.6 mol%. Based on the genotypic and phenotypic characteristics, strain SM30 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed with the type strain SM30 (=NBRC 111955=DSM 102969).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Lanthanum , methanol dehydrogenase and Oharaeibacter diazotrophicus gen. nov., sp. nov.
© 2017 IUMS
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2017-03-01
2024-04-19
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Oharaeibacter diazotrophicus gen. nov., sp. nov., a diazotrophic and facultatively methylotrophic bacterium, isolated from rice rhizosphere
Int J Syst Evol Microbiol 67, 576 (2017); https://doi.org/10.1099/ijsem.0.001660
/content/journal/ijsem/10.1099/ijsem.0.001660
/content/journal/ijsem/10.1099/ijsem.0.001660
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