RT Journal Article SR Electronic(1) A1 Gobeli Brawand, Stefanie A1 Cotting, Kerstin A1 Gómez-Sanz, Elena A1 Collaud, Alexandra A1 Thomann, Andreas A1 Brodard, Isabelle A1 Rodriguez-Campos, Sabrina A1 Strauss, Christian A1 Perreten, VincentYR 2017 T1 Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 3 SP 621 OP 626 DO https://doi.org/10.1099/ijsem.0.001673 PB Microbiology Society, SN 1466-5034, AB Gram-stain-positive cocci were isolated from miscellaneous sites of the skin of healthy dogs as well as from infection sites in dogs. The closest relative by sequencing of the 16S rRNA gene was Macrococcus caseolyticus with 99.7 % sequence identity, but compared with M. caseolyticus , the novel strains shared only 90.8 to 93.5 % DNA sequence identity with cpn60, dnaJ, rpoB and sodA partial genes, respectively. The novel strains also exhibited differential phenotypic characteristics from M. caseolyticus , and the majority displayed a visible haemolysis on sheep blood agar, while M. caseolyticus did not have any haemolytic activity. They generated different matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS spectral profiles compared with the other species of the genus Macrococcus . Furthermore, strain KM 45013T shared only 53.7 % DNA–DNA relatedness with the type strain of M. caseolyticus , confirming that they do not belong to the same species. The DNA G+C content of strain KM 45013T was 36.9 mol%. The most abundant fatty acids were C14 : 0, C18 : 3ω6c (6, 9, 12) and C16 : 0 n alcohol. MK-6 was the menaquinone type of KM 45013T. Cell-wall structure analysis revealed that the peptidoglycan type was A3α l-Lys–Gly2–l-Ser. Based on genotypic and chemotaxonomic characteristics, we propose to classify these strains within a novel species of the genus Macrococcus for which the name Macrococcus canis sp. nov. is proposed. The type strain is KM 45013T (=DSM 101690T=CCOS 969T=CCUG 68920T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001673