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Volume 67, Issue 3

sp. nov., isolated from oil-contaminated soil Free

Abstract

During the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Biratnagar, Morang, Nepal, a yellow-coloured, Gram-staining-negative, aerobic, non-motile, and rod-shaped bacterium, designated strain C-5-3, was isolated. This strain was characterized taxonomically by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain C-5-3 belonged to the genus and was closely related to DY46 (98.19 % sequence similarity), AG1-2 (98.15 % sequence similarity), BCRC 17412 (98.14 % sequence similarity), THG C4-1 (97.73 % sequence similarity) and DSM 25574 (97.60 % sequence similarity). The predominant respiratory quinone was menaquinone-6, and phosphatidylethanolamine was the major polar lipid. The predominant fatty acids of strain C-5-3 were iso-C, summed feature 3 (Cω7 and/or Cω6), iso-C 3-OH and summed feature 9 (iso- Cω9 and/or C 10-methyl). The genomic DNA G+C content of this novel strain was 38.6 mol%. The DNA–DNA relatedness between strain C-5-3 and JCM 19801, DSM 26898, KACC 13400, KACC 16985 and DSM 25574 was 53.3, 42.7, 47.3, 33.0 and 28.0 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain C-5-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C-5-3 (=KEMB 9005-411=KACC 18907=JCM 31469).

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Keyword(s): Bacteroidetes , Chryseobacterium nepalense sp. nov. , Flavobacteriaceae and oil-contaminated soil
© 2017 IUMS
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2017-03-01
2024-04-16
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Chryseobacterium nepalense sp. nov., isolated from oil-contaminated soil
Int J Syst Evol Microbiol 67, 646 (2017); https://doi.org/10.1099/ijsem.0.001680
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