@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001681, author = "Harbison, Austin B. and Price, Laiken E. and Flythe, Michael D. and Bräuer, Suzanna L.", title = "Micropepsis pineolensis gen. nov., sp. nov., a mildly acidophilic alphaproteobacterium isolated from a poor fen, and proposal of Micropepsaceae fam. nov. within Micropepsales ord. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "4", pages = "839-844", doi = "https://doi.org/10.1099/ijsem.0.001681", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001681", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "bacteria", keywords = "Micropepsales", keywords = "peatland", keywords = "Alphaproteobacteria", abstract = "An obligately anaerobic, mildly acidophilic, fermentative alphaproteobacterium, designated strain CS4T, was isolated from an acidic, oligotrophic (nutrient poor) poor fen located near Pineola, NC, USA. Cultures contained Gram-negative, slightly curved, non-motile, non-spore-forming, non-prosthecate rods (0.1–0.4 µm in diameter and 0.4–4 µm long, depending, in part, on the growth substrate). Growth optima were 35 °C (range 15–35 °C), pH 5.6 (range of 5.0–6.8), and with 0–50 mM added NaCl (range, 0–100 mM added NaCl). The culture fermented cellobiose, d-glucose, d-mannose, fructose, galactose, glycerol, lactose, maltose, peptone, sucrose, trehalose and xylose. Respiratory growth was not detected. Major fatty acids were C18 : 1ω7c, C19 : 0 cyclo ω8c, C14 : 0 and C16 : 0. The G+C content of the DNA was 61.9±0.3 mol%. The two most closely related species phylogenetically, Rhizomicrobium palustre A48T (AB081581) and Rhizomicrobium electricum Mfc52T (AB365487) shared 94 and 93 % SSU rRNA gene sequence identity, respectively, to that of strain CS4T. Lower SSU rRNA gene sequence identities resulted from pairwise comparisons with members of the order ‘ Rhizobiales’ (85–88 %) or Sphingomonadales (85–86 %). These findings all support the classification of strain CS4T as representative of a novel genus, family and order of Alphaproteobacteria . The type strain of the species pineolensis within the genus Micropepsis , family Micropepsaceae and order Micropepsales is CS4T (=JCM 30711T=ATCC BAA-2724T).", }