RT Journal Article SR Electronic(1) A1 Xia, Zhan-Feng A1 Luo, Xiao-Xia A1 Wan, Chuan-Xing A1 Zhang, Li-LiYR 2017 T1 Saccharopolyspora aidingensis sp. nov., an actinomycete isolated from a salt lake JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 3 SP 687 OP 691 DO https://doi.org/10.1099/ijsem.0.001696 PB Microbiology Society, SN 1466-5034, AB A novel halophilic actinomycete strain, designated TRM 46074T, was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074T was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H4), MK-10(H8) and MK-10(H4). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074T belongs to the genus Saccharopolyspora . Strain TRM 46074T had 16S rRNA gene sequence similarity of 99.6 % with the closest described species, Saccharopolyspora ghardaiensis H53T. DNA–DNA hybridization between strain TRM 46074T and Saccharopolyspora ghardaiensis H53T showed 58.6 % relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074T is considered to represent a novel species in the genus Saccharopolyspora , for which the name Saccharopolyspora aidingensis sp. nov. is proposed. The type strain is TRM 46074T (=CCTCC AA 2012014T=JCM 30185T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001696