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Volume 67, Issue 3

gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of as comb. nov. Free

Abstract

A bacterial strain, designated 5GHs33-3, was isolated from greenhouse soil collected from Yongin region, Gyeonggi province, South Korea. The strain was an aerobic, Gram-stain-positive, flagellated, rod-shaped bacterium. Strain 5GHs33-3 grew at 4–37 °C (optimally at 28–30 °C), pH 6.0–10.0 (optimally at pH 7.0) and with 0–7 % (w/v) NaCl (optimally with 0 %). The 16S rRNA gene sequence analysis revealed that strain 5GHs33-3 had high sequence similarity with EGI 60002 (98.4 %), CF5-4 (96.4 %) and DSM 3133 (96.2 %), and less than 95.5 % sequence similarity against all the other species with validly published names. The phylogenetic tree revealed that strain 5GHs33-3 formed a robust independent monophyletic line with EGI 60002. The predominant fatty acids of strain 5GHs33-3 were anteiso-C and iso-C. The only quinone was MK-8(H). Polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown phosphoglycolipid and unknown lipids. The peptidoglycan type was A4, with ornithine as the diagnostic diamino acid and an interpeptide bridge comprising -Glu. The DNA G+C content is 69.0 mol%. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses, strain 5GHs33-3 represents a novel species of a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is 5GHs33-3 (=KACC 16542=NBRC 111006). We also propose the reclassification of as comb. nov. (type strain EGI 60002=JCM 19624=KACC 17839=KCTC 29256).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinotlea suaedae , menaquinone , Pseudactinotalea suaedae and Pseudactinotalea terrae
© 2017 IUMS
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2017-03-01
2024-04-23
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Pseudactinotalea terrae gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov.
Int J Syst Evol Microbiol 67, 704 (2017); https://doi.org/10.1099/ijsem.0.001701
/content/journal/ijsem/10.1099/ijsem.0.001701
/content/journal/ijsem/10.1099/ijsem.0.001701
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