1887

Abstract

Two novel anaerobic, mesophilic, biohydrogen-producing bacteria, designated strains ZGM211 and G1, were isolated from lake sediment. 16S rRNA and ATP synthase beta subunit ( ) gene sequences and phylogenetic analysis of strains ZGM211 and G1 revealed an affiliation to the genus (cluster I of the clostridia), with as the closest characterized species, showing the same sequence similarity of 96.4 % to the type strain (98.9 % between the two isolates). Cells of the two strains were rod shaped. Growth occurred at 20–45 °C, pH 4.0–8.0 and NaCl concentrations up to 2 % (w/v). Grown on glucose, the main fermentation products were H, CO, acetate and butyrate. The major fatty acids were C and C. The DNA G+C contents of strains ZGM211 and G1 were 40.7 and 41.5 mol%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic differences, strains ZGM211 (=CICC 24070=BCRC 80950) and G1 (=CICC 24069=BCRC 80949) are proposed as the type strains of novel species of the genus with the names sp. nov. and sp. nov., respectively.

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2017-03-01
2024-03-29
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