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f Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., two phylogenetically closely related hydrogen-producing species isolated from lake sediment

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Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., two phylogenetically closely related hydrogen-producing species isolated from lake sediment, Page 1 of 1

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  • Two novel anaerobic, mesophilic, biohydrogen-producing bacteria, designated strains ZGM211 and G1, were isolated from lake sediment. 16S rRNA and ATP synthase beta subunit (atp D) gene sequences and phylogenetic analysis of strains ZGM211 and G1 revealed an affiliation to the genus Clostridium sensu stricto (cluster I of the clostridia), with Clostridium acetobutylicum as the closest characterized species, showing the same sequence similarity of 96.4 % to the type strain (98.9 % between the two isolates). Cells of the two strains were rod shaped. Growth occurred at 20–45 °C, pH 4.0–8.0 and NaCl concentrations up to 2 % (w/v). Grown on glucose, the main fermentation products were H2, CO2, acetate and butyrate. The major fatty acids were C14 : 0 and C16 : 0. The DNA G+C contents of strains ZGM211 and G1 were 40.7 and 41.5 mol%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic differences, strains ZGM211 (=CICC 24070=BCRC 80950) and G1 (=CICC 24069=BCRC 80949) are proposed as the type strains of novel species of the genus Clostridium with the names Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., respectively.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strains ZGM211 and G1 are KT000268 and KT824779, respectively, and for the partial atpD gene sequences are KY087895 and KY087896.

  • Two supplementary figures and three supplementary tables are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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2019-02-15
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