@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001706, author = "Checinska Sielaff, Aleksandra and Kumar, Rajendran Mathan and Pal, Deepika and Mayilraj, Shanmugam and Venkateswaran, Kasthuri", title = "Solibacillus kalamii sp. nov., isolated from a high-efficiency particulate arrestance filter system used in the International Space Station", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "4", pages = "896-901", doi = "https://doi.org/10.1099/ijsem.0.001706", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001706", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "International Space Station", keywords = "HEPA", keywords = "16S rRNA", keywords = "endospores", keywords = "Solibacillus", abstract = "A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterial strain, designated ISSFR-015T, was isolated from a high-efficiency particulate arrestance filter in the International Space Station and was characterized by polyphasic taxonomy. A comparative analysis of the 16S rRNA gene sequence (1494 bp) of strain ISSFR-015T showed highest similarity to Solibacillus isronensis B3W22T (98.9 %), followed by Solibacillus silvestris HR3-23T (98.6 %) and Bacillus cecembensis PN5T (96.7 %). DNA–DNA hybridization analysis revealed that the DNA relatedness values of strain ISSFR-015T with other closely related species were in the range of 41–47 % [ S. silvestris MTCC 10789T (47 %), S. isronensis MTCC 7902T (41 %) and B. cecembensis MTCC 9127T (43 %)]. The DNA G+C content of strain ISSFR-015T was 45.4 mol%. The major fatty acids were iso-C15 : 0 (45.2 %) and C17 : 1ω10c (12.1 %). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and one unknown phospholipid. The isoprenoid quinones present in strain ISSFR-015T were MK-7 (86.8 %), MK-6 (11.6 %) and MK-8 (1.0 %). The peptidoglycan type of the cell wall was A4α l-Lys-d-Glu. Based on the phylogenetic analysis, strain ISSFR-015T belongs to the genus Solibacillus . The polyphasic taxonomic data, including low DNA–DNA hybridization values, and the chemotaxonomic analysis confirmed that strain ISSFR-015T represents a novel species, for which the name Solibacillus kalamii sp. nov. is proposed. The type strain for this proposed species is ISSFR-015T (=NRRL B-65388T=DSM 101595T).", }