@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001723, author = "Lin, Shih-Yao and Hameed, Asif and Liu, You-Cheng and Hsu, Yi-Han and Hung, Mei-Hua and Lai, Wei-An and Young, Chiu-Chung", title = "Ruficoccus amylovorans gen. nov., sp. nov., an amylolytic and nitrate-reducing diazotroph of the family Puniceicoccaceae", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "4", pages = "956-962", doi = "https://doi.org/10.1099/ijsem.0.001723", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001723", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "strain CC-MHH0563T", keywords = "Ruficoccus amylovorans", keywords = "Puniceicoccaceae", abstract = "A novel amylolytic, nitrate-reducing and diazotrophic bacterium, designated strain CC-MHH0563T, isolated from a fermenter was assessed for its taxonomic status using a polyphasic approach. Cells of strain CC-MHH0563T were Gram-staining-negative, catalase- and oxidase-positive, mesophilic and aerobic cocci, which produced reddish nondiffusible pigments. Growth was observed at 15–37 °C (optimal 25 °C), pH 6.0–8.0 (optimal pH 7.0) and salinity of 0–3 % (w/v). Strain CC-MHH0563T showed highest pairwise 16S rRNA gene sequence similarity to members of the genera Cerasicoccus (89.3–89.5 %), Coraliomargarita (87.8 %), Pelagicoccus (85.8–86.4 %) and Puniceicoccus (87.9 %), and established a discrete taxonomic lineage during phylogenetic analysis. The major fatty acids found in strain CC-MHH0563T were C14 : 0, anteiso-C15 : 0, C16 : 0, C17 : 0, C18 : 0 and C18 : 1ω9c. The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unknown aminolipids and two unknown aminophospholipids. The polyamine pattern showed a predominance of spermidine and a minor amount of cadaverine. The DNA G+C content was 57.4 mol% and the predominant quinone system was menaquinone-7. The low 16S rRNA gene sequence similarity values (<90.0 %) and a distinct phylogenetic clustering clearly distinguished strain CC-MHH0563T from other representatives of the family Puniceicoccaceae . Based on the discrete phylogenetic, phenotypic and chemotaxonomic traits together with the results of comparative 16S rRNA gene sequence analysis, strain CC-MHH0563T is considered to represent a novel genus and species of the family Puniceicoccaceae , for which the name Ruficoccus amylovorans gen. nov., sp. nov. is proposed. The type strain of the type species is CC-MHH0563T (=BCRC 80918T=JCM 31066T).", }