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f Noviherbaspirillum agri sp. nov., isolated from reclaimed grassland soil, and reclassification of Herbaspirillum massiliense (Lagier et al., 2014) as Noviherbaspirillum massiliense comb. nov.

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Noviherbaspirillum agri sp. nov., isolated from reclaimed grassland soil, and reclassification of Herbaspirillum massiliense (Lagier et al., 2014) as Noviherbaspirillum massiliense comb. nov., Page 1 of 1

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  • A straw-coloured, Gram-staining-negative, aerobic, motile and rod-shaped bacterium, designated strain K-1-15, was isolated from reclaimed grassland soil from Biratnagar, Morang, Nepal. This strain was non-spore-forming, catalase-negative and oxidase-positive. It was able to grow at 10–45 °C, pH 6.5–9.5 and 0–1.5 % (w/v) NaCl concentration. This strain was taxonomically characterized by a polyphasic approach. Based on the results of 16S rRNA gene sequence analysis, K-1-15 formed a distinct lineage within the family Oxalobacteraceae and was most closely related to members of the genera Herbaspirillum (96.99–95.34 % sequence similarity), Noviherbaspirillum (96.72–95.45 % sequence similarity) and Paraherbaspirillum (95.85 % sequence similarity). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major fatty acids of K-1-15 were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16  :   0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C10 : 0 3-OH, and iso-C16 : 0. The genomic DNA G+C content of this novel strain was 65.2 mol %. The DNA–DNA relatedness between K-1-15 and Herbaspirillum massiliense DSM 25712 and Noviherbaspirillum soli LMG 26149 were 18.3 and 13.7 % repectively. On the basis of the results of morphological, physiological, chemotaxonomic and phylogenetic analyses, K-1-15 represents a novel species of the genus Noviherbaspirillum in the family Oxalobacteraceae , for which the name Noviherbaspirillum agri sp. nov. is proposed. The type strain is K-1-15 (=KEMB 9005-422=KACC 18909=JCM 31463). Based on new data obtained in this study, we also propose the reclassification of Herbaspirillum massiliense as Noviherbaspirillum massiliense comb. nov. (type strain JC206=CSUR P159=DSM 25712).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain K-25 1-15 is KX129821.

  • Five supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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