@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001761, author = "Glaeser, Stefanie P. and Tobias, Nicholas J. and Thanwisai, Aunchalee and Chantratita, Narisara and Bode, Helge B. and Kämpfer, Peter", title = "Photorhabdusluminescens subsp. namnaonensis subsp. nov., isolated from Heterorhabditisbaujardi nematodes", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "4", pages = "1046-1051", doi = "https://doi.org/10.1099/ijsem.0.001761", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001761", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "taxonomy", keywords = "nematode", keywords = "Photorhabdus luminescens subsp. namnaonensis", abstract = "A lightly yellowish-pigmented, oxidase-negative bacterial strain (PB45.5T) isolated from the Nam Nao district of Phetchabun in central Thailand was investigated to determine its taxonomic position. Cells of the isolate showed a rod shaped appearance. The strain stained Gram-negative. Strain PB45.5T shared highest 16S rRNA gene sequence similarity with the type strains of Photorhabdus luminescens subsp. akhurstii (99.2 %) and Photorhabdus luminescens subsp. hainanensis (99.1 %) and lower similarities to all other Photorhabdus luminescens subspecies (<98.0 %). Multilocus sequence analysis (MLSA) based on concatenated partial recA, dnaN, gltX, gyrB and infB gene sequences confirmed the affiliation obtained by 16S rRNA gene sequence analysis but showed a clear distinction of PB45.5T from the closest related type strains. Strain PB45.5T shared only 96.9 % sequence similarity (concatenated nucleotide sequences) with P. luminescens subsp. akhurstii FRG04T and 96.8 % with P. luminescens subsp. hainanensis C8404T. The fatty acid profile of the strain consisted of the major fatty acids C14 : 0, C16 : 0, C17 : 0 cyclo, C16 : 1ω7c and/or iso-C15 : 0 2-OH, and C18 : 1 ω7c. The MLSA results and the differential biochemical and chemotaxonomic properties showed that strain PB45.5T represents a novel P. luminescens subspecies, for which the name Photorhabdus luminescens subsp. namnaonensis subsp. nov. (type strain PB45.5T=LMG 29915T=CCM 8729T) is proposed.", }