@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001765, author = "Li, Jiahong and Huang, Jing and Liao, Shuijiao and Wang, Gejiao", title = "Pseudaminobacter manganicus sp. nov., isolated from sludge of a manganese mine", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "5", pages = "1589-1594", doi = "https://doi.org/10.1099/ijsem.0.001765", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001765", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "manganese mine", keywords = "Pseudaminobacter", abstract = "A Gram-staining-negative, aerobic, non-motile, capsule-forming and rod-shaped bacterium, designated JH-7T, was isolated from sludge of a manganese mine. The 16S rRNA gene sequence of JH-7T showed highest similarities to those of Pseudaminobacter salicylatoxidans BN12T (97.4 %), Mesorhizobiumthiogangeticum SJTT (97.0 %) and Pseudaminobacter defluvii THI 051T (96.5 %). Phylogenetic trees clustered JH-7T together with P. salicylatoxidans BN12T and P. defluvii THI 051T. The DNA–DNA hybridization values between JH-7T and P. salicylatoxidans DSM 6986T and between JH-7T and M . thiogangeticum DSM 17097T were 34.8 and 20.1 %, respectively. The major fatty acids of JH-7T (>10 %) were C18 : 1ω7c, C19 : 0cyclo ω8c and C16 : 0. The genomic DNA G+C content was 61.6 mol%. The polyamines of JH-7T were sym-homospermidine (83 %) and putrescine (17 %), and the respiratory quinone was ubiquinone-10. The major polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids and two unidentified lipids. Compared with the members of the genera Pseudaminobacter and Mesorhizobium , JH-7T showed some unique physiological and biochemical characters, such as being negative for H2S production, hydrolysis of Tween 40 and Tween 60, esterase lipase (C8) activity and assimilation of d-ribose and positive for acid production from d-galactose and assimilation of d-fructose. On the basis of the results of the polyphasic taxonomic analysis, JH-7T was considered to represent a novel species of the genus Pseudaminobacter , for which the name Pseudaminobacter manganicus sp. nov. is proposed. The type strain is JH-7T (=KCTC 52258T=CCTCC AB 2016107T).", }