RT Journal Article SR Electronic(1) A1 Feng, Wei-Wei A1 Wang, Tian-Tian A1 Bai, Juan-Luan A1 Ding, Peng A1 Xing, Ke A1 Jiang, Ji-Hong A1 Peng, Xue A1 Qin, ShengYR 2017 T1 Glutamicibacter halophytocola sp. nov., an endophytic actinomycete isolated from the roots of a coastal halophyte, Limonium sinense JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 5 SP 1120 OP 1125 DO https://doi.org/10.1099/ijsem.0.001775 PB Microbiology Society, SN 1466-5034, AB A novel actinobacterium, designated KLBMP 5180T, was isolated from the surface-sterilized root of a coastal halophyte, Limonium sinense, collected from the city of Lianyungang, Jiangsu Province, eastern China. The isolate was Gram-stain-positive, aerobic and non-motile. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine. The predominant menaquinone was MK-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phospholipid, one unidentified glycolipid and two unidentified lipids. anteiso-C15 : 0 and iso-C16 : 0 were the major cellular fatty acids. The DNA G+C content of strain KLBMP 5180T was 60.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KLBMP 5180T belongs to the genus Glutamicibacter and was related most closely to Glutamicibacter nicotianae DSM 20123T (99.3 % similarity), Glutamicibacterarilaitensis Re117T (99.3 %) and Glutamicibacter mysorens LMG 16219T (99.1 %); similarity to other type strains of the genus Glutamicibacter was lower than 98.5 %. However, DNA–DNA relatedness values between strain KLBMP 5180T, G . nicotianae DSM 20123T, G. arilaitensis Re117T and G. mysorens LMG 16219T were 47.5±2.6, 51.3±3.1 and 41.2±4.3 %, respectively. The combination of DNA–DNA hybridization, phylogenetic, phenotypic and chemotaxonomic data supported the suggestion that strain KLBMP 5180T represents a novel species of the genus Glutamicibacter, for which the name Glutamicibacter halophytocola sp. nov. is proposed. The type strain is KLBMP 5180T (=DSM 101718T=KCTC 39692T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001775