@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001777, author = "Khan, Inam Ullah and Hussain, Firasat and Tian, Ye and Habib, Neeli and Xian, Wen-Dong and Jiang, Zhao and Amin, Arshia and Yuan, Chang-Guo and Zhou, En-Min and Zhi, Xiao-Yang and Li, Wen-Jun", title = "Tibeticola sediminis gen. nov., sp. nov., a thermophilic bacterium isolated from a hot spring", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "5", pages = "1133-1139", doi = "https://doi.org/10.1099/ijsem.0.001777", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001777", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Tibeticola sediminis gen. nov., sp. nov.,", keywords = "family Comamonadaceae", abstract = "Two closely related thermophilic bacterial strains, designated YIM 73013T and YIM 73008, were isolated from a sediment sample collected from a hot spring in Tibet, western Tibet province, China. The taxonomic positions of the two isolates were investigated using a polyphasic approach. The novel isolates were Gram-stain-negative, aerobic, short-rod-shaped and motile by means of a polar flagellum. They were oxidase- and catalase-positive and were able to grow at 30–55 °C (optimum, 37–45 °C), at pH 6.0–8.0 (optimum, pH 7.0) and with NaCl tolerance up to 1 % (w/v). Phylogenetic analyses based on 16S rRNA gene sequences showed that strains YIM 73013T and YIM 73008 formed a distinct lineage with respect to closely related genera in the family Comamonadaceae and shared highest 16S rRNA gene sequences similarities with Acidovorax caeni R-24608T (96.3 and 96.4 %, respectively). The respiratory quinone was ubiquinone-8 (Q-8) and the major cellular fatty acids observed were C17 : 1ω6c, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The genomic DNA G+C contents of strains YIM 73013T and YIM 73008 were 68.7 and 68.3 mol%, respectively. Based on the morphological, phylogenetic and chemotaxonomic results, the two isolates represent a novel species in a new genus, for which the name Tibeticola sediminis gen. nov., sp. nov. is proposed. The type strain of Tibeticola sediminis is YIM 73013T (=DSM 101684T=KCTC 42873T).", }