@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001811, author = "Ding, Jiun-Yan and Chen, Sheng-Chung and Lai, Mei-Chin and Liao, Tsai-Ling", title = "Haloterrigena mahii sp. nov., an extremely halophilic archaeon from a solar saltern", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "5", pages = "1333-1338", doi = "https://doi.org/10.1099/ijsem.0.001811", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001811", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Haloterrigena", keywords = "Solar saltern", keywords = "Genome-to-Genome Distance Analysis (GGDA)", keywords = "Polyhydroxyalkanoate (PHA)", keywords = "Average Nucleotide Identity (ANI)", keywords = "Haloarchaea", abstract = "A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3–10 µm in length. The optimum growth conditions were 3.5–5.0 M NaCl, 45–55 °C, and pH range of 6.5–8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13T was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and rpoB′ genes revealed that strain H13 was most closely related to Haloterrigena saccharevitans AB14T (16S rRNA gene sequence similarity: 99.51 %; rpoB′ sequence similarity: 96.19 %) and Haloterrigena thermotolerans PR5T (99.11 %; 95.50 %). Strain H13T showed low genome relatedness values with Htg. saccharevitans AB14T and Htg. thermotolerans PR5T based on estimated average nucleotide identity (ANI; 92.59 and 91.68 %, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00 %, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13T represents a novel species of the genus Haloterrigena , for which the name Haloterrigena mahiisp. nov. is proposed. The type strain is H13T (=BCRC 910151T=NBRC 111885T).", }