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Volume 67, Issue 11

sp. nov., a moderately halophilic bacterium isolated from a tidal flat Free

Abstract

Strain Hb3 was isolated from a tidal flat in Jeollabuk-do Gunsan, Republic of Korea. Cells were Gram-stain-negative, oxidase- and catalase-positive, rod-shaped and motile. The strain grew optimally at 25–35 °C, at pH 6.0–6.5 and with 3.0–10.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Hb3 belonged to the genus . Strain Hb3 was related most closely to Al12 (98.6 % 16S rRNA gene sequence similarity), M29 (98.6 %) and AJ275 (98.4 %). Moreover, multilocus sequence analysis using the , and genes supported the phylogenetic position of strain Hb3. The genomic G+C content of strain Hb3 was 67.9 mol%. DNA–DNA hybridization values for strain Hb3 versus Al12, M29 and AJ275 were 38.0, 54.5 and 47.4 %, respectively. The major quinone was ubiquinone Q-9 and the major fatty acids were Cω7, summed feature 3 (Cω6 and/or Cω7), C and C cyclo 8. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, amino lipid, six unidentified phospholipids and an unidentified lipid comprised the polar lipid profile. On the basis of the data presented in this report, strain Hb3 represents a novel species of the genus . The name sp. nov. is proposed for this novel species. The type strain is Hb3 (=KCTC 52253=JCM 31415).

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  • Accepted:
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Keyword(s): Halomonas aestuarii , halophilic , novel species , polyphasic and tidal flat
© 2017 IUMS
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2017-11-01
2024-04-19
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Halomonas aestuarii sp. nov., a moderately halophilic bacterium isolated from a tidal flat
Int J Syst Evol Microbiol 67, 4298 (2017); https://doi.org/10.1099/ijsem.0.001824
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