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Volume 67, Issue 6

sp. nov. and sp. nov., isolated from bovine raw milk Free

Abstract

Five strains, designated WS 4672, WS 4998, WS 4992, WS 4997 and WS 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. The most similar species on the basis of 16S rRNA gene sequences were IAM 1603, CIP 105469 and CIP 105460 showing 99.7–99.6 % similarity. Using gene sequences LMG 17761 (93.3 %) was most closely related to strain WS 4672 and CIP 105460 to strain WS 4992 (93.3 %). The five strains could be differentiated from their closest relatives and from each other by phenotypic and chemotaxonomic characterization and ANIb values calculated from draft genome assemblies. ANIb values of strains WS 4992 and WS4671 to the closest relatives are lower than 90 %. The major cellular polar lipids of both strains are phosphatidylethanolamine, phosphatidylglycerol, a phospholipid and diphosphatidylglycerol, and their major quinone is Q-9. The DNA G+C content of strains WS 4992 and WS 4672 were 60.0  and 59.7  mol%, respectively. Based on these genotypic and phenotypic traits two novel species of the genus are proposed: sp. nov. [with type strain WS 4992 (=DSM 29167=LMG 28435) and the additional strains WS 4997 and WS 5000], and sp. nov. [with type strain WS 4672 (=DSM 29164=LMG 28439) and additional strain WS 4998].

  • Received:
  • Accepted:
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Keyword(s): peptidase , Pseudomonas and raw milk
© 2017 IUMS
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2017-06-01
2024-04-24
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Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk
Int J Syst Evol Microbiol 67, 1656 (2017); https://doi.org/10.1099/ijsem.0.001836
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