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f Actinomyces vulturis sp. nov., isolated from Gyps himalayensis

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  • Authors: Xiangli Meng1​ ,  Shan Lu1​ ,  Yiting Wang1​,2​ ,  Xin-He Lai3​ ,  Yumeng Wen1​ ,  Dong Jin1​ ,  Jing Yang1​ ,  Xiangning Bai1​ ,  Gui Zhang1​ ,  Ji Pu1​ ,  Riuting Lan4​ , Jianguo Xu1​,5​
    • VIEW AFFILIATIONS
    • 1​ 1​State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, PR China 2​ 2​Institute for Immunization and Prevention, Beijing Center for Diseases Prevention and Control, Beijing 100013, PR China 3​ 3​Institute of Translational Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, PR China 4​ 4​School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia 5​ 5​Shanghai Institute for Emerging and Remerging Infectious Diseases, Shanghai Public Health Clinical Center, Jinshan, Shanghai, PR China
  • *Correspondence: Jianguo Xu, [email protected]
  • First Published Online: 20 June 2017, International Journal of Systematic and Evolutionary Microbiology 67: 1720-1726, doi: 10.1099/ijsem.0.001851
  • Subject: New Taxa - Actinobacteria
  • Received:
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  • Two strains of Gram-stain-positive, facultatively anaerobic, non-spore-forming short rods (VUL7 and VUL8) were isolated from rectal swabs of Old World vultures, namely Gyps himalayensis, in Tibet-Qinghai Plateau, China. Optimal growth occurred at 37 °C, pH 6–7, with 1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences classified the two strains to the genus Actinomyces , with highest 16S rRNA gene sequence similarity (95 %) to type strains of Actinomyces haliotis , Actinomyces radicidentis and Actinomyces urogenitalis . The major cellular fatty acids were C18 : 1ω9c and C16 : 0. MK-10(H4) was the major respiratory quinone. The genomic DNA G+C content of the isolate was 54.4 mol%. DNA–DNA hybridization values with the most closely related species of the genus Actinomyces was 24.6 %. The two strains can be differentiated from the most closely related species such as A. haliotis , A. radicidentis, A. graevenitzii and A. urogenitalis by a list of carbohydrate fermentations and enzyme activities. On the basis of physiological, biochemical and phylogenetic analysis, strains VUL7 and VUL8 represent novel species of the genus Actinomyces , for which the name Actinomyces vulturis sp. nov. is proposed. The type strain is VUL7 (=CGMCC 4.7366=DSM 103437).

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains VUL7 and VUL8 are KX360125 and KX389558, respectively. The GenBank/EMBL/DDBJ accession number for the draft genome sequence of strain VUL7 is LZRK00000000.

  • Two supplementary figures and one supplementary table are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

Erratum

An erratum has been published for this content:
Corrigendum: Actinomyces vulturis sp. nov., isolated from Gyps himalayensis
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