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Volume 67, Issue 6

sp. nov., isolated from an agricultural floodplain soil, and emended description of the genus Free

Abstract

A novel slow-growing bacterial strain designated as AW305 was isolated from an agricultural floodplain soil located in Mashare, Kavango region, Namibia. Cells stained Gram-negative, were non-motile, non-spore-forming, coccoid to rod-shaped and did not form a capsule. Colonies were yellow-pigmented, but flexirubin-type pigments were not detected. AW305 had an aerobic chemo-organoheterotrophic metabolism, using a narrow spectrum of carbon sources for growth, with preference for complex protein substrates, organic acids and amino acids. AW305 was able to grow at 15–40 °C, pH 5.3–8.3 and in the presence of up to 0.25 % (w/v) NaCl. 16S rRNA gene sequence comparison showed that AW305 belonged to the genus (family ). Its closest relatives were GCR0105 (97.0 %), Gsoil 492 (93.6 %) and Gsoil 643 (93.2 %). DNA–DNA hybridization experiments corroborated that AW305 represents an independent genomospecies. The genomic DNA G+C content was 57.6 mol%. Major fatty acids were iso-C G, iso-C, Cω5, iso-C 3-OH and summed feature 3 (Cω7 and/or iso-C 2-OH). The predominant respiratory quinone was MK-7, albeit minor amounts of MK-6 were also detected. The polar lipids comprised major amounts of phosphatidylethanolamine and minor amounts of two unidentified lipids, an unidentified phospholipid, an unidentified glycolipid and an unidentified aminoglycophospholipid. On the basis of the polyphasic characterization, strain AW305 represents a novel species of the genus for which the name sp. nov. is proposed, with the type strain AW305 (=DSM 100282=LMG 29416).

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Keyword(s): Bacteroidetes , Chitinophagaceae and Flaviaesturariibacter
© 2017 IUMS
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2017-06-01
2024-04-19
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Flaviaesturariibacter luteus sp. nov., isolated from an agricultural floodplain soil, and emended description of the genus Flaviaesturariibacter
Int J Syst Evol Microbiol 67, 1727 (2017); https://doi.org/10.1099/ijsem.0.001854
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