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f Salinicola tamaricis sp. nov., a heavy-metal-tolerant, endophytic bacterium isolated from the halophyte Tamarix chinensis Lour

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Salinicola tamaricis sp. nov., a heavy-metal-tolerant, endophytic bacterium isolated from the halophyte Tamarix chinensis Lour, Page 1 of 1

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  • A Gram-stain-negative, rod-shaped bacterium, strain F01, was isolated from leaves of Tamarix chinensis Lour. The isolate grew optimally at 30 °C, at pH 7.0 and with 5.0 % (w/v) NaCl, and showed a high tolerance to manganese, lead, nickel, ferrous ions and copper ions. The major fatty acids were C18 : 1ω7c and C16 : 0, and the predominant respiratory quinone was Q-9. Polar lipids were dominated by diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminoglycolipids and phospholipids. The DNA G+C content was 65.8 %. Based on multilocus phylogenetic analysis, strain F01 belonged to the genus Salinicola , with highest 16S rRNA gene sequence similarity to Salinicola peritrichatus CGMCC 1.12381 (97.7 %). The level of DNA–DNA hybridization between strain F01 and closely related Salinicola strains was well below 70 %. According to the phenotypic, genetic and chemotaxonomic data, strain F01 is considered to represent a novel species in the genus Salinicola , for which the name Salinicola tamaricis sp. nov. is proposed. The type strain is F01 (=CCTCC AB 2015304=KCTC 42855).

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB and rpoD gene sequences of strain F01 are KT343976, KT613295 and KX459385, respectively.

  • Eight supplementary figures and one supplementary table are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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2019-02-22
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